Characterization of Antibiotic Actinomycin D Isolated from Streptomyces parvulus Collected from Marine Sponge of Barrang Lompo Island, Makassar, Indonesia.

Infectious disorders drug targets Pub Date : 2025-01-01 DOI:10.2174/0118715265306848240719061135

Herlina Rante, Gemini Alam, Yosi Bayu Murti, Alimuddin Ali

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Abstract

Background: Microorganisms associated with sea sponges have proven to be good natural product resources that are biologically active and pharmaceutically important.

Objective: This research aimed to identify actinomycetes related to a sponge from Barranglompo Island Makassar and the antibacterial compounds.

Methods: Identification of actinomycetes was based on molecular characterization of sequence gen16S rRNA. The antibacterial compound was separated using vacuum liquid chromatography and preparative Thin Layer Chromatography (TLC). The structure determination was done based on spectroscopy 1H-NMR, 13C-NMR, 2D NMR, and mass spectra.

Results: Molecular characterization showed that actinomycetes strain BLP 20 had the closest relationship with Streptomyces parvulus and Uncultured Streptomyces sp. with a similarity value of 83%. The results obtained from the characterization of antibacterial compounds based on spectroscopic data indicate that these compounds lead to Actinomycin D.

Conclusion: Characterization and identification of Strain 20 / BLP by molecular phylogenetic analysis of 16S rRNA sequences revealed the closest relationship with Uncultured Streptomyces sp and S. parvulus with a similarity value of 83%, which indicated a new species. The structure of the active compound isolated from actinomycetes strain 20 / BLP leads to Actinomycin D.

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从印度尼西亚望加锡 Barrang Lompo 岛海洋海绵中采集的链霉菌中分离出的抗生素放线菌素 D 的特征。

背景：与海海绵相关的微生物已被证明是具有生物活性和重要药用价值的良好天然产品资源：本研究旨在鉴定与望加锡 Bar-ranglompo 岛海绵有关的放线菌及其抗菌化合物：放线菌的鉴定基于基因 16S rRNA 序列的分子特征。使用真空液相色谱法和制备型薄层色谱法（TLC）分离抗菌化合物。根据 1H-NMR、13C-NMR、2D NMR 和质谱进行了结构测定：分子鉴定结果表明，放线菌菌株 BLP 20 与副链霉菌（Streptomyces parvulus）和未培养链霉菌（Uncultured Streptomyces sp.根据光谱数据对抗菌化合物进行表征的结果表明，这些化合物可产生放线菌素 D：通过 16S rRNA 序列的分子系统进化分析，对菌株 20 / BLP 进行了特征描述和鉴定，发现其与未培养的 Streptomy-ces sp 和 S. parvulus 的关系最为密切，相似度为 83%，这表明这是一个新物种。从放线菌菌株 20 / BLP 中分离出的活性化合物的结构为放线菌素 D。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Infectious disorders drug targets

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