The yellowhorn MYB transcription factor MYB30 is required for wax accumulation and drought tolerance.

IF 3.5 2区农林科学 Q1 FORESTRY

Tree physiology Pub Date : 2024-10-03 DOI:10.1093/treephys/tpae111

Xiaojuan Liu, Zhuo Ban, Yingying Yang, Huihui Xu, Yifan Cui, Chenxue Wang, Quanxin Bi, Haiyan Yu, Libing Wang

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引用次数: 0

Abstract

Yellowhorn (Xanthoceras sorbifolium Bunge) is an economically important tree species in northern China, mainly distributed in arid and semi-arid areas where water resources are scarce. Drought affects its yield and the expansion of its suitable growth area. It was found that the wax content in yellowhorn leaves varied significantly among different germplasms, which had a strong correlation with the drought resistance of yellowhorn. In this study, XsMYB30 was isolated from 'Zhongshi 4' of yellowhorn, a new highly waxy variety. DAP-Seq technology revealed that the pathways associated with fatty acids were significantly enriched in the target genes of XsMYB30. Moreover, the results of electrophoretic mobility shift assay, yeast one hybrid assay and dual-luciferase assay demonstrated that XsMYB30 could directly and specifically bind with the promoters of genes involved in wax biosynthesis (XsFAR4, XsCER1 and XsKCS1), lipid transfer (XsLTPG1 and XsLTP1) and fatty acid synthesis (XsKASIII), thus enhancing their expression. In addition, the overexpression of XsMYB30 in poplar promoted the expression levels of these target genes and increased the wax deposition on poplar leaves leading to a notable improvement in the plant's ability to withstand drought. These findings indicate that XsMYB30 is an important regulatory factor in cuticular wax biosynthesis and the drought resistance of yellowhorn.

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黄角 MYB 转录因子 MYB30 是蜡质积累和抗旱所必需的。

黄角树（Xanthoceras sorbifolium）是中国北方重要的经济树种，主要分布在水资源匮乏的干旱和半干旱地区。干旱影响其产量和适宜生长区域的扩大。研究发现，黄角树不同种质资源叶片蜡质含量差异显著，这与黄角树的抗旱性有很大关系。本研究从高蜡质新品种黄角'中实 4 号'中分离出 XsMYB30。DAP-Seq 技术显示，与脂肪酸相关的通路在 XsMYB30 的靶基因中显著富集。此外，电泳迁移试验、酵母一杂交试验和双荧光素酶试验的结果表明，XsMYB30能直接与蜡质生物合成（XsFAR4、XsCER1和XsKCS1）、脂质转移（XsLTPG1和XsLTP1）和脂肪酸合成（XsKASIII）相关基因的启动子结合，从而提高它们的表达量。此外，在杨树中过表达 XsMYB30 能促进这些靶基因的表达水平，并增加杨树叶片上的蜡沉积，从而显著提高植物的抗旱能力。这些研究结果表明，XsMYB30 是黄杨角质蜡生物合成和抗旱性的重要调控因子。

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来源期刊

Tree physiology 农林科学-林学

CiteScore

7.10

自引率

7.50%

发文量

133

审稿时长

1 months

期刊介绍： Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.