Multi-site enhancement of osteogenesis: peptide-functionalized GelMA hydrogels with three-dimensional cultures of human dental pulp stem cells.

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2024-08-10 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae090
Leyi Liang, Shuze Wang, Xiyue Zhang, Tao Yan, Xiyun Pan, Yuzhong Gao, Xing Zhang, Qiang Wang, Liu Qu
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引用次数: 0

Abstract

Human dental pulp stem cells (hDPSCs) have demonstrated greater proliferation and osteogenic differentiation potential in certain studies compared to other types of mesenchymal stem cells, making them a promising option for treating craniomaxillofacial bone defects. However, due to low extracting concentration and long amplifying cycles, their access is limited and utilization rates are low. To solve these issues, the principle of bone-forming peptide-1 (BFP1) in situ chemotaxis was utilized for the osteogenic differentiation of hDPSCs to achieve simultaneous and synergistic osteogenesis at multiple sites. BFP1-functionalized gelatin methacryloyl hydrogel provided a 3D culture microenvironment for stem cells. The experimental results showed that the 3D composite hydrogel scaffold constructed in this study increased the cell spread area by four times compared with the conventional GelMA scaffold. Furthermore, the problems of high stem cell dosage and low rate of utilization were alleviated by orchestrating the programmed proliferation and osteogenic differentiation of hDPSCs. In vivo, high-quality repair of critical bone defects was achieved using hDPSCs extracted from a single tooth, and multiple 'bone island'-like structures were successfully observed that rapidly induced robust bone regeneration. In conclusion, this study suggests that this kind of convenient, low-cost, island-like osteogenesis strategy involving a low dose of hDPSCs has great potential for repairing craniomaxillofacial critical-sized bone defects.

多部位增强成骨:多肽功能化 GelMA 水凝胶与人牙髓干细胞的三维培养。
与其他类型的间充质干细胞相比,人牙髓干细胞(hDPSCs)在某些研究中显示出更大的增殖和成骨分化潜力,使其成为治疗颅颌面骨缺损的一种有前途的选择。然而,由于提取浓度低、扩增周期长,其获取途径有限,利用率低。为了解决这些问题,研究人员利用骨形成肽-1(BFP1)的原位趋化原理,对hDPSCs进行成骨分化,以实现多部位同步协同成骨。BFP1功能化明胶甲基丙烯酰水凝胶为干细胞提供了三维培养微环境。实验结果表明,本研究构建的三维复合水凝胶支架与传统的GelMA支架相比,细胞扩散面积增加了四倍。此外,通过协调 hDPSCs 的程序化增殖和成骨分化,干细胞用量大、利用率低的问题也得到了缓解。在体内,利用从单颗牙齿中提取的hDPSCs实现了关键骨缺损的高质量修复,并成功观察到多个 "骨岛 "状结构,可迅速诱导骨再生。总之,这项研究表明,这种使用低剂量 hDPSCs 的便捷、低成本、岛状骨生成策略在修复颅颌面关键性骨缺损方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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