Diffusible signal factor (DSF)-mediated quorum sensing modulates swarming in Xanthomonas albilineans.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2024-08-27 DOI:10.1094/PHYTO-03-24-0076-R

MeiLin Li, YiXue Bao, WenHan Chen, YiSha Li, JinXia Du, Abdullah Khan, Muhammad Tahir Khan, Charles A Powell, BaoShan Chen, MuQing Zhang

{"title":"Diffusible signal factor (DSF)-mediated quorum sensing modulates swarming in Xanthomonas albilineans.","authors":"MeiLin Li, YiXue Bao, WenHan Chen, YiSha Li, JinXia Du, Abdullah Khan, Muhammad Tahir Khan, Charles A Powell, BaoShan Chen, MuQing Zhang","doi":"10.1094/PHYTO-03-24-0076-R","DOIUrl":null,"url":null,"abstract":"Xanthomonas spp. are plant pathogens known for significantly impacting crop yields. Among them, Xanthomonas albilineans (Xal) is notable for colonizing the xylem and causing sugarcane leaf scald disease. This study employed homologous recombination to mutate quorum sensing (QS) regulatory genes (rpf) to investigate their role in Xal pathogenicity. Deletions of rpfF (ΔrpfF), rpfC (ΔrpfC), and rpfG (ΔrpfG) led to reduced swarming, growth, and virulence. However, DSF supplementation restored swarming and growth in the ΔrpfF mutant. Deleting rpfC, rpfG, and rpfF also reduced twitching motility and affected Type IV Pilus (T4P) expression. Transcriptomic analysis revealed that ΔrpfF positively regulates flagellar genes. DSF supplementation in ΔrpfF (ΔrpfF-DSF) modulated the expression of flagellar, chemotaxis, and T4P genes. These findings elucidate the DSF-mediated swarming pathway in Xal and provide valuable insights into its regulatory mechanisms.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-03-24-0076-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Xanthomonas spp. are plant pathogens known for significantly impacting crop yields. Among them, Xanthomonas albilineans (Xal) is notable for colonizing the xylem and causing sugarcane leaf scald disease. This study employed homologous recombination to mutate quorum sensing (QS) regulatory genes (rpf) to investigate their role in Xal pathogenicity. Deletions of rpfF (ΔrpfF), rpfC (ΔrpfC), and rpfG (ΔrpfG) led to reduced swarming, growth, and virulence. However, DSF supplementation restored swarming and growth in the ΔrpfF mutant. Deleting rpfC, rpfG, and rpfF also reduced twitching motility and affected Type IV Pilus (T4P) expression. Transcriptomic analysis revealed that ΔrpfF positively regulates flagellar genes. DSF supplementation in ΔrpfF (ΔrpfF-DSF) modulated the expression of flagellar, chemotaxis, and T4P genes. These findings elucidate the DSF-mediated swarming pathway in Xal and provide valuable insights into its regulatory mechanisms.

查看原文本刊更多论文

可扩散信号因子（DSF）介导的法定量感应可调节白化黄单胞菌的群集。

黄单胞菌属是植物病原体，以严重影响作物产量而闻名。其中，白化黄单胞菌（Xal）以在木质部定殖并引起甘蔗叶烫病而闻名。本研究采用同源重组法突变法定量感应（QS）调控基因（rpf），研究它们在Xal致病性中的作用。rpfF（ΔrpfF）、rpfC（ΔrpfC）和rpfG（ΔrpfG）的缺失导致蜂群、生长和毒力降低。然而，补充 DSF 能恢复 ΔrpfF 突变体的蜂群和生长。删除 rpfC、rpfG 和 rpfF 也会降低抽动运动能力并影响 IV 型 Pilus（T4P）的表达。转录组分析表明，ΔrpfF 对鞭毛基因有正向调节作用。在ΔrpfF中补充DSF（ΔrpfF-DSF）可调节鞭毛基因、趋化基因和T4P基因的表达。这些发现阐明了Xal中DSF介导的蜂拥途径，并为其调控机制提供了宝贵的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.