A novel mutation in non-constitutive lycopene beta cyclase (CstLcyB2a) from Crocus sativus modulates carotenoid/apocarotenoid content, biomass and stress tolerance in plants.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-08-27 DOI:10.1007/s00425-024-04515-x
Javid Ahmad Mir, Arvind Kumar Yadav, Deepika Singh, Nasheeman Ashraf
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引用次数: 0

Abstract

Main conclusion: Mutation at A126 in lycopene-β-cyclase of Crocus (CstLcyB2a) sterically hinders its binding of δ-carotene without affecting lycopene binding, thereby diverting metabolic flux towards β-carotene and apocarotenoid biosynthesis. Crocus sativus, commonly known as saffron, has emerged as an important crop for research because of its ability to synthesize unique apocarotenoids such as crocin, picrocrocin and safranal. Metabolic engineering of the carotenoid pathway can prove a beneficial strategy for enhancing the quality of saffron and making it resilient to changing climatic conditions. Here, we demonstrate that introducing a novel mutation at A126 in stigma-specific lycopene-β-cyclase of Crocus (CstLcyB2a) sterically hinders its binding of δ-carotene, but does not affect lycopene binding, thereby diverting metabolic flux towards β-carotene formation. Thus, A126L-CstLcyB2a expression in lycopene-accumulating bacterial strains resulted in enhanced production of β-carotene. Transient expression of A126L-CstLcyB2a in C. sativus stigmas enhanced biosynthesis of crocin. Its stable expression in Nicotiana tabacum enhanced β-branch carotenoids and phyto-hormones such as abscisic acid (ABA) and gibberellic acids (GA's). N. tabacum transgenic lines showed better growth performance and photosynthetic parameters including maximum quantum efficiency (Fv/Fm) and light-saturated capacity of linear electron transport. Exogenous application of hormones and their inhibitors demonstrated that a higher ratio of GA4/ABA has positive effects on biomass of wild-type and transgenic plants. Thus, these findings provide a platform for the development of new-generation crops with improved productivity, quality and stress tolerance.

Abstract Image

番石榴非组成型番茄红素β环化酶(CstLcyB2a)的新型突变可调节植物的类胡萝卜素/类胡萝卜素含量、生物量和抗逆性。
主要结论番红花的番茄红素-β-环化酶(CstLcyB2a)中 A126 处的突变在不影响番茄红素结合的情况下立体地阻碍了其δ-胡萝卜素的结合,从而使代谢通量转向β-胡萝卜素和类胡萝卜素的生物合成。番红花(俗称藏红花)是一种重要的研究作物,因为它能够合成独特的类胡萝卜素,如藏红花苷(crocin)、小藏红花苷(picrocrocin)和藏红花醛(safranal)。类胡萝卜素途径的代谢工程可证明是提高藏红花品质并使其适应不断变化的气候条件的有益策略。在这里,我们证明在番红花柱头特异性番茄红素-β-环化酶(CstLcyB2a)的 A126 处引入一个新的突变,会固态地阻碍其与δ-胡萝卜素的结合,但不会影响番茄红素的结合,从而使代谢通量转向β-胡萝卜素的形成。因此,在番茄红素积累细菌菌株中表达 A126L-CstLcyB2a 可提高 β-胡萝卜素的产量。在 C. sativus 柱头中瞬时表达 A126L-CstLcyB2a 能增强黄腐素的生物合成。A126L-CstLcyB2a 在烟草中的稳定表达增强了β-类胡萝卜素和植物激素,如脱落酸(ABA)和赤霉素(GA's)。N.tabacum转基因品系表现出更好的生长性能和光合参数,包括最大量子效率(Fv/Fm)和线性电子传递的光饱和能力。外源应用激素及其抑制剂表明,较高的 GA4/ABA 比率对野生型和转基因植物的生物量有积极影响。因此,这些发现为开发具有更高的生产力、品质和抗逆性的新一代作物提供了一个平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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