Cold mediates maize root hair developmental plasticity via epidermis-specific transcriptomic responses.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Yaping Zhou, Mauritz Leonard Sommer, Annika Meyer, Danning Wang, Alina Klaus, Tyll Stöcker, Caroline Marcon, Heiko Schoof, Georg Haberer, Chris-Carolin Schön, Peng Yu, Frank Hochholdinger
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Abstract

Cold stress during early development limits maize (Zea mays L.) production in temperate zones. Low temperatures restrict root growth and reprogram gene expression. Here, we provide a systematic transcriptomic landscape of maize primary roots, their tissues, and cell types in response to cold stress. The epidermis exhibited a unique transcriptomic cold response, and genes involved in root hair formation were dynamically regulated in this cell type by cold. Consequently, activation of genes involved in root hair tip growth contributed to root hair recovery under moderate cold conditions. The maize root hair defective mutants roothair defective 5 (rth5) and roothair defective 6 (rth6) displayed enhanced cold tolerance with respect to primary root elongation. Furthermore, DEHYDRATION RESPONSE ELEMENT-BINDING PROTEIN 2.1 (DREB2.1) was the only member of the dreb subfamily of AP2/EREB transcription factor genes upregulated in primary root tissues and cell types but exclusively downregulated in root hairs upon cold stress. Plants overexpressing dreb2.1 significantly suppressed root hair elongation after moderate cold stress. Finally, the expression of rth3 was regulated by dreb2.1 under cold conditions, while rth6 transcription was regulated by DREB2.1 irrespective of the temperature regime. We demonstrated that dreb2.1 negatively regulates root hair plasticity at low temperatures by coordinating the expression of root hair defective genes in maize.

寒冷通过表皮特异性转录组反应介导玉米根毛发育的可塑性
温带地区玉米(Zea mays L.)生长早期的冷胁迫限制了其产量。低温限制了根系的生长并重塑了基因表达。在这里,我们提供了玉米主根、其组织和细胞类型对冷胁迫反应的系统转录组图谱。表皮表现出独特的转录组冷响应,参与根毛形成的基因在这种细胞类型中受到冷的动态调控。因此,参与根毛顶端生长的基因的激活有助于根毛在中等寒冷条件下的恢复。玉米根毛缺陷突变体根毛缺陷 5(rth5)和根毛缺陷 6(rth6)在主根伸长方面表现出更强的耐寒性。此外,脱水反应元件结合蛋白 2.1(dreb2.1)是 AP2/EREB 转录因子 dreb 亚家族基因中唯一在主根组织和细胞类型中上调的成员,但在冷胁迫时仅在根毛中下调。过表达 dreb2.1 的植株在中度冷胁迫后会显著抑制根毛的伸长。最后,在寒冷条件下,rth3 的表达受 dreb2.1 的调控,而 rth6 的转录受 dreb2.1 的调控,与温度条件无关。我们证明,dreb2.1 通过协调玉米根毛缺陷基因的表达,在低温条件下负向调控根毛的可塑性。
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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