Antioxidant and Skin-Whitening Efficacy of a Novel Decapeptide (DP, KGYSSYICDK) Derived from Fish By-Products.

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2024-08-20 DOI:10.3390/md22080374

Sung-Gyu Lee, Jin-Woo Hwang, Hyun Kang

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Abstract

The skin is vulnerable to damage from ultraviolet rays and oxidative stress, which can lead to aging and pigmentation issues. This study investigates the antioxidant and whitening efficacy of a decapeptide (DP, KGYSSYICDK) derived from marine fish by-products and evaluates its potential as a new skin-whitening agent. DP demonstrated high antioxidant activity, showing comparable or superior performance to Vitamin C (Vit. C) in ferric reducing antioxidant power (FRAP) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. In hydrogen peroxide (H₂O₂)-treated HaCaT cells, DP increased cell viability and reduced reactive oxygen species (ROS) generation. Furthermore, DP inhibited tyrosinase activity and decreased melanin production in α-melanocyte stimulating hormone (α-MSH)-induced B16F10 melanoma cells in a dose-dependent manner. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that DP reduces the mRNA expression of MITF, tyrosinase, and MC1R, thus suppressing melanin production. DP exhibits strong binding interactions with multiple amino acid residues of tyrosinase, indicating potent inhibitory effects on the enzyme. These results suggest that DP possesses significant antioxidant and whitening properties, highlighting its potential as a skin-whitening agent. Future research should focus on optimizing DP's structure and exploring structure-activity relationships.

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从鱼类副产品中提取的新型十肽（DP，KGYSSYICDK）的抗氧化和美白功效

皮肤很容易受到紫外线和氧化应激的伤害，从而导致老化和色素沉着问题。本研究调查了一种从海洋鱼类副产品中提取的十肽（DP，KGYSSYICDK）的抗氧化和美白功效，并评估了其作为新型皮肤美白剂的潜力。DP 具有很高的抗氧化活性，在铁还原抗氧化能力（FRAP）和 2,2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除试验中的表现与维生素 C（Vit.在过氧化氢（H2O2）处理的 HaCaT 细胞中，DP 可提高细胞活力并减少活性氧（ROS）的生成。此外，DP还能抑制酪氨酸酶的活性，并以剂量依赖的方式减少α-黑色素细胞刺激素（α-MSH）诱导的B16F10黑色素瘤细胞中黑色素的生成。反转录聚合酶链反应（RT-PCR）分析表明，DP 可降低 MITF、酪氨酸酶和 MC1R 的 mRNA 表达，从而抑制黑色素的生成。DP 与酪氨酸酶的多个氨基酸残基有很强的结合相互作用，表明其对酪氨酸酶有很强的抑制作用。这些结果表明，DP 具有显著的抗氧化和美白特性，凸显了其作为皮肤美白剂的潜力。未来的研究应侧重于优化 DP 的结构和探索结构-活性关系。

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.

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