Panicum maximum Jacq. mediated green synthesis of silver nanoparticles: synthesis, characterization, and biological activities supported by molecular docking.

IF 4.5 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Heba W Alhamdi, Hanan Khalaf Anazi, Fatma Alzahraa Mokhtar, Seham S Elhawary, Serag Eldin I Elbehairi, Mohammad Y Alfaifi, Ali A Shati, Lamiaa I Fahmy, Engy Elekhnawy, Afnan Hassan, Walaa A Negm, Sherif Ashraf Fahmy, Nabil Selim
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Abstract

This study uses the aerial parts of Panicum maximum total extract (PMTE) to synthesize silver nanoparticles (AgNPs) in an environmentally friendly manner. TEM, SEM, FTIR, X-ray powder diffraction (XRD), Zeta potential, UV, and FTIR were used to characterize the green silver nanoparticles (PM-AgNPs). PM-AgNPs were evaluated as anticancer agents compared to (PMTE) against breast (MCF-7), lung (A549), and ovary adenocarcinoma (SKOV3) human tumour cells. The antibacterial activity of AgNPs was assessed against Staphylococcus aureus isolates. The PM-AgNPs had an absorbance of 418 nm, particle size of 15.18 nm, and zeta potential of -22.4 mV, ensuring the nanosilver's stability. XRD evaluated the crystallography nature of the formed PM-AgNPs. The cytotoxic properties of PM-AgNPs on MCF-7 and SKOV 3 were the strongest, with IC50s of 0.13 ± 0.015 and 3.5 ± 0.5 g/ml, respectively, as compared to A549 (13 ± 3.2 µg/mL). The increase in the apoptotic cells was 97.79 ± 1.61 and 96.6 ± 1.91% for MCF-7 and SKOV3 cell lines, respectively. PM-AgNPs were found to affect the membrane integrity and membrane permeability of 50 and 43.75% of the tested isolates, respectively. Also, PM-AgNPs have recorded a reduction in the biofilm formation of S. aurues. These results suggest using PM-AgNPs to treat breast and ovarian cancers.

Panicum maximum Jacq.介导的银纳米粒子的绿色合成:分子对接支持的合成、表征和生物活性。
本研究利用最大连翘的气生部分总提取物(PMTE)以环保的方式合成银纳米粒子(AgNPs)。利用 TEM、SEM、FTIR、X 射线粉末衍射 (XRD)、Zeta 电位、紫外线和 FTIR 对绿色银纳米粒子(PM-AgNPs)进行了表征。评估了 PM-AgNPs 与 PMTE 相比对乳腺癌(MCF-7)、肺癌(A549)和卵巢腺癌(SKOV3)人类肿瘤细胞的抗癌作用。评估了 AgNPs 对金黄色葡萄球菌分离物的抗菌活性。PM-AgNPs 的吸光度为 418 nm,粒径为 15.18 nm,Zeta 电位为 -22.4 mV,确保了纳米银的稳定性。XRD 评估了所形成的 PM-AgNPs 的晶体学性质。PM-AgNPs 对 MCF-7 和 SKOV 3 的细胞毒性最强,IC50 值分别为 0.13 ± 0.015 和 3.5 ± 0.5 g/ml,而对 A549 的 IC50 值为 13 ± 3.2 µg/mL。MCF-7 和 SKOV3 细胞系的凋亡细胞增加率分别为 97.79 ± 1.61% 和 96.6 ± 1.91%。研究发现,PM-AgNPs 分别影响了 50% 和 43.75% 受测分离物的膜完整性和膜渗透性。此外,PM-AgNPs 还能减少 S. aurues 的生物膜形成。这些结果建议使用 PM-AgNPs 治疗乳腺癌和卵巢癌。
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来源期刊
Artificial Cells, Nanomedicine, and Biotechnology
Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
10.90
自引率
0.00%
发文量
48
审稿时长
20 weeks
期刊介绍: Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.
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