Dried Zingiber officinale Alleviates Dehydroepiandrosterone-Induced Ovarian Granulosa Cell Apoptosis via the Reactive Oxygen Species-Mediated Pathway

IF 3.5 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuan Liu, Xin Luo, Keran Jia, Shuang Liu, Yongqiu Zeng, Xiyuan Lin, Xinyue Lin, Ying Wan, Lishang Liao, Hongwei Su, Jingyan Yi, Jinshan Xing
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Abstract

Polycystic ovary syndrome (PCOS), a prevalent reproductive endocrine disorder, frequently coincides with insulin resistance, lipid dysregulation, and cellular apoptosis. In this study, we aimed to evaluate the therapeutic potential of Dried Zingiber officinale (DZO), renowned for its antibacterial, antiviral, anti-inflammatory, and antioxidant properties, in the context of PCOS. To this end, we induced a PCOS mouse model through the administration of dehydroepiandrosterone (DHEA) and a high-fat diet (HFD), followed by DZO treatment to assess its effects on ovarian pathology, insulin resistance, and hormonal imbalances. The anti-apoptotic effect of DZO on PCOS ovarian granulosa cells was confirmed through network pharmacological analysis, TUNEL staining, FITC-PI staining, and protein blotting. Notably, DZO treatment significantly alleviated ovarian pathological changes in PCOS mice and normalized hormone levels, including testosterone, estradiol, and progesterone/follicle-stimulating hormone ratios. Furthermore, our findings confirmed the anti-apoptotic effect of DZO on PCOS ovarian granulosa cells. Mechanistically, DZO primarily exerted its therapeutic effects in PCOS by inhibiting apoptosis induced by the accumulation of reactive oxygen species (ROS). In conclusion, our study demonstrates the promising therapeutic role of DZO in the management of obese PCOS patients, particularly in reversing ROS-mediated apoptosis in ovarian granulosa cells.

Abstract Image

干姜通过活性氧诱导途径缓解脱氢表雄酮诱导的卵巢颗粒细胞凋亡
多囊卵巢综合征(PCOS)是一种常见的生殖内分泌疾病,常伴有胰岛素抵抗、脂质失调和细胞凋亡。在本研究中,我们旨在评估因具有抗菌、抗病毒、抗炎和抗氧化特性而闻名的干姜(DZO)对多囊卵巢综合征的治疗潜力。为此,我们通过服用脱氢表雄酮(DHEA)和高脂饮食(HFD)诱导出多囊卵巢综合征小鼠模型,然后用 DZO 治疗,以评估其对卵巢病理学、胰岛素抵抗和激素失衡的影响。通过网络药理学分析、TUNEL染色、FITC-PI染色和蛋白质印迹,证实了DZO对多囊卵巢综合征卵巢颗粒细胞的抗凋亡作用。值得注意的是,DZO 治疗明显缓解了 PCOS 小鼠卵巢的病理变化,并使激素水平恢复正常,包括睾酮、雌二醇和孕酮/卵泡刺激素比率。此外,我们的研究结果还证实了 DZO 对多囊卵巢综合征卵巢颗粒细胞的抗凋亡作用。从机理上讲,DZO 主要通过抑制活性氧(ROS)积累诱导的细胞凋亡来发挥其对多囊卵巢综合征的治疗作用。总之,我们的研究表明,DZO 在治疗肥胖型多囊卵巢综合征患者方面具有良好的治疗作用,尤其是在逆转 ROS 介导的卵巢颗粒细胞凋亡方面。
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来源期刊
Journal of Food Biochemistry
Journal of Food Biochemistry 生物-生化与分子生物学
CiteScore
7.80
自引率
5.00%
发文量
488
审稿时长
3.6 months
期刊介绍: The Journal of Food Biochemistry publishes fully peer-reviewed original research and review papers on the effects of handling, storage, and processing on the biochemical aspects of food tissues, systems, and bioactive compounds in the diet. Researchers in food science, food technology, biochemistry, and nutrition, particularly based in academia and industry, will find much of great use and interest in the journal. Coverage includes: -Biochemistry of postharvest/postmortem and processing problems -Enzyme chemistry and technology -Membrane biology and chemistry -Cell biology -Biophysics -Genetic expression -Pharmacological properties of food ingredients with an emphasis on the content of bioactive ingredients in foods Examples of topics covered in recently-published papers on two topics of current wide interest, nutraceuticals/functional foods and postharvest/postmortem, include the following: -Bioactive compounds found in foods, such as chocolate and herbs, as they affect serum cholesterol, diabetes, hypertension, and heart disease -The mechanism of the ripening process in fruit -The biogenesis of flavor precursors in meat -How biochemical changes in farm-raised fish are affecting processing and edible quality
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