A New Ex Vivo Model to Study Cardiac Fibrosis in Whole Mouse Hearts

IF 8.4 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS
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引用次数: 0

Abstract

Fibrosis is a characteristic of many cardiac diseases for which no effective treatment exists. We have developed an ex vivo flow system, which allows induction of cardiac fibrosis in intact adult mouse hearts. Lineage-tracing studies indicated that the collagen-producing myofibroblasts originated from the resident fibroblasts. The extent of fibrosis was flow rate dependent, and pharmacological inhibition of the transforming growth factor beta signaling pathway prevented fibrosis. Therefore, in this powerful system, the cellular and molecular mechanisms underlying cardiac fibrosis can be studied. In addition, new targets can be tested on organ level for their ability to inhibit fibrosis.

研究小鼠全心心脏纤维化的新型体内外模型
纤维化是许多心脏疾病的特征,但目前尚无有效的治疗方法。我们开发了一种体外流动系统,可在完整的成年小鼠心脏中诱导心脏纤维化。系谱追踪研究表明,产生胶原蛋白的肌成纤维细胞来源于常驻成纤维细胞。纤维化的程度与流速有关,药物抑制转化生长因子β信号通路可防止纤维化。因此,在这个功能强大的系统中,可以研究心脏纤维化的细胞和分子机制。此外,还可以在器官水平上测试新靶点抑制纤维化的能力。
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来源期刊
JACC: Basic to Translational Science
JACC: Basic to Translational Science CARDIAC & CARDIOVASCULAR SYSTEMS-
CiteScore
14.20
自引率
1.00%
发文量
161
审稿时长
16 weeks
期刊介绍: JACC: Basic to Translational Science is an open access journal that is part of the renowned Journal of the American College of Cardiology (JACC). It focuses on advancing the field of Translational Cardiovascular Medicine and aims to accelerate the translation of new scientific discoveries into therapies that improve outcomes for patients with or at risk for Cardiovascular Disease. The journal covers thematic areas such as pre-clinical research, clinical trials, personalized medicine, novel drugs, devices, and biologics, proteomics, genomics, and metabolomics, as well as early phase clinical trial methodology.
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