Characteristics and nontargeted metabolomics analysis of anammox granular sludge under short-term exposure to polypropylene and polylactic acid microplastics

Abstract

A significant quantity of microplastics (MPs) is concealed within biological sludge. Current research predominantly examines the effects of non-biodegradable MPs on traditional sludge. However, the influence of biodegradable MPs on the functional microbial activity of anaerobic ammonium oxidation granular sludge (AnGS) remains inadequately explored. This study specifically investigated the effects of non-biodegradable polypropylene (PP) MPs and biodegradable polylactic acid (PLA) MPs on AnGS under short-term stress. The study found that PP MPs inhibited nitrogen removal performance, reducing nitrogen removal efficiency by 1.14% (100 mesh) and 5.77% (1000 mesh) respectively, along with decreased hydrazine dehydrogenase (HDH) activity. Conversely, PLA promoted denitrification performance, increasing efficiency by 8.21% (100 mesh) and 6.54% (1000 mesh). In response to MPs-induced environmental stress, the secretion of extracellular polymeric substances (EPS) increased in all experimental groups. Additionally, non-targeted metabolomic analysis revealed a decrease in the abundance of KEGG pathways related to carbon and amino acid metabolism in the experimental groups, while the enrichment of terephthalate and benzamide in the PLA groups significantly impacted its process. These findings offered valuable insights into the impact of MPs on anaerobic ammonium oxidation (anammox) and could potentially enhance its application.