Immunocytochemistry of bone marrow aspirates: a tool in the diagnosis of feline leukemia virus infection in cats

IF 0.8 4区农林科学 Q4 PATHOLOGY

Journal of Comparative Pathology Pub Date : 2024-08-26 DOI:10.1016/j.jcpa.2024.07.007

Thierry G. de Cristo , Giovana Biezus , Aline R. Maciel , Geanice Ledo , Mayara Vavassori , Ubirajara M. da Costa , Luiz C. Miletti , Renata A. Casagrande

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引用次数: 0

Abstract

Feline leukemia virus (FeLV) is a highly debilitating cat pathogen due to its ability to cause many pathological changes. Therefore, identifying the virus directly in bone marrow can be a highly relevant diagnostic tool even in the absence of viraemia. The aim of this study was to compare the diagnostic efficiency of immunocytochemistry (ICC) of bone marrow aspirates with enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Blood samples were collected from 188 cats and separated into aliquots of whole blood for nested PCR using the U3 LTR region and the gag gene of FeLV-A as reference and serum for detection of the p27 antigen by ELISA. Bone marrow samples from these cats were placed on silanized slides for anti-FeLV ICC using gp70 as primary antibody. A total of 28.2% of the cats tested for FeLV were positive in at least one of the tests, with 26.6% positive by PCR, 18.1% by ICC and 11.2% by ELISA. Cohen's kappa agreement test revealed moderate agreement between ELISA and PCR results and substantial agreement between ICC and ELISA and between ICC and PCR. The results indicated that ICC of bone marrow is an efficient novel diagnostic test for FeLV infection.

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骨髓抽吸物的免疫细胞化学：诊断猫感染猫白血病病毒的工具

猫白血病病毒（FeLV）可引起多种病理变化，是一种严重影响猫咪健康的病原体。因此，即使在没有病毒血症的情况下，直接鉴定骨髓中的病毒也是一种非常有用的诊断工具。本研究旨在比较骨髓抽吸物免疫细胞化学（ICC）与酶联免疫吸附试验（ELISA）和聚合酶链反应（PCR）的诊断效率。采集了 188 只猫的血样，并将其分离成等分全血，以 FeLV-A 的 U3 LTR 区域和 gag 基因为参考进行巢式 PCR，并用 ELISA 检测血清中的 p27 抗原。将这些猫的骨髓样本放在硅烷化载玻片上，使用 gp70 作为第一抗体进行抗 FeLV ICC 检测。在接受 FeLV 检测的猫中，28.2% 的猫在至少一项检测中呈阳性，其中 26.6% 的猫在 PCR 检测中呈阳性，18.1% 的猫在 ICC 检测中呈阳性，11.2% 的猫在 ELISA 检测中呈阳性。Cohen's kappa 一致性检验显示，ELISA 和 PCR 结果之间的一致性为中等，ICC 和 ELISA 之间以及 ICC 和 PCR 之间的一致性很高。结果表明，骨髓 ICC 是一种有效的新型 FeLV 感染诊断检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Comparative Pathology 生物-病理学

CiteScore

1.60

自引率

0.00%

发文量

208

审稿时长

50 days

期刊介绍： The Journal of Comparative Pathology is an International, English language, peer-reviewed journal which publishes full length articles, short papers and review articles of high scientific quality on all aspects of the pathology of the diseases of domesticated and other vertebrate animals. Articles on human diseases are also included if they present features of special interest when viewed against the general background of vertebrate pathology.