A transcriptomics-based analysis of mechanisms involved in the sex-dependent effects of diazepam on zebrafish

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-08-23 DOI:10.1016/j.aquatox.2024.107063

Yibing Zhang , Yanhong Shi , Jie Tang , Kun Chen , Min Wu , Xiangyang Wu , Xuchun Qiu

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Abstract

Diazepam (DZP) is a universally detected emerging pollutant in aquatic ecosystems. Although the sex-dependent effects of DZP on fish have been properly established, the underlying mechanisms remain unclear. In this study, zebrafish of both sexes were separately exposed to DZP (8 μg/L) for 21 days, and the alteration of the behaviors, brain amino acid neurotransmitter contents, and transcriptomic profiles were investigated. Although DZP exposure showed a sedative effect on both sexes, significantly reduced cumulative duration of high mobility and willingness to encounter the opposite sex were only observed in females. However, DZP significantly enhanced the brain levels of glutamate and glutamine in males but not in females. Transcriptome analysis identified more different expression genes (DEGs) in females (322 up-regulated and 311 down-regulated) than in males (138 up-regulated genes and 38 down-regulated). The DEGs in both sexes were significantly enriched in the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway of the synaptic vesicle cycle, indicating a possible pathway for the sedative effects of DZP on zebrafish. DZP exhibited different or even opposing regulatory patterns on gene expression in the brains of females and males, providing some insights into its sex-dependent impacts on the behaviors and brain neurotransmitter contents in zebrafish. Moreover, enrichment analysis also suggested that DZP exposure may affect the oocyte maturation in female zebrafish, which highlights the need to study its reproductive and transgenerational toxicity to fish species.

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基于转录组学的地西泮对斑马鱼的性别依赖效应机制分析

地西泮（DZP）是水生生态系统中普遍检测到的一种新出现的污染物。虽然 DZP 对鱼类的影响与性别有关，但其潜在机制仍不清楚。本研究将雌雄斑马鱼分别暴露于 DZP（8 μg/L）中 21 天，研究了斑马鱼的行为、脑氨基酸神经递质含量和转录组图谱的变化。虽然DZP对雌雄动物都有镇静作用，但只有雌性动物的高移动性和与异性相遇意愿的累积持续时间显著减少。然而，DZP能显著提高雄性大脑中谷氨酸和谷氨酰胺的水平，而雌性则没有。转录组分析发现，雌性（322个上调基因和311个下调基因）的不同表达基因（DEGs）多于雄性（138个上调基因和38个下调基因）。雌雄斑马鱼的 DEGs 在 KEGG（京都基因与基因组百科全书）的突触囊泡循环途径中都有显著的富集，这表明 DZP 对斑马鱼的镇静作用可能有一个途径。DZP对雌性和雄性斑马鱼大脑基因表达的调控模式不同，甚至截然相反，这有助于了解DZP对斑马鱼行为和大脑神经递质含量的性别依赖性影响。此外，富集分析还表明，暴露于 DZP 可能会影响雌性斑马鱼卵母细胞的成熟，这凸显了研究其对鱼类物种的生殖毒性和跨代毒性的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.