Circulating MicroRNAs as a biomarker signature of perinatal asphyxia.

IF 2.3 4区医学 Q2 PEDIATRICS

Pediatrics and Neonatology Pub Date : 2024-08-20 DOI:10.1016/j.pedneo.2024.05.002

Yin-Hsuan Lai, Yi-Fang Tu, Chia-Huei Chen, Jui-Hsing Chang, Chyong-Hsin Hsu, Man-Yau Ho, Liang-Ti Huang, Nan-Chang Chiu, Che-Sheng Ho, Jinn-Li Wang, Ruei-Ming Chen

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引用次数: 0

Abstract

Aim: This study aimed to explore whether microRNAs (miRNAs) could serve as biomarkers of perinatal asphyxia and whether they were correlated with severity of brain magnetic resonance imaging.

Methods: We prospectively enrolled 26 full-term newborns, including 10 with perinatal asphyxia and 16 healthy controls. Plasma samples were collected at 0-6 h and 7 days of age. Encephalopathy was classified according to modified Sarnat staging. Magnetic resonance imaging was performed in surviving infants within 30 days of birth, and a score was established. We used next-generation sequencing to explore differentially expressed miRNAs, which were then further validated using quantitative reverse transcription real-time polymerase chain reaction (RT-PCR).

Results: A significantly lower expression of miR-486-5p was found at 0-6 h of age in the asphyxiated newborns compared with the healthy controls (p = 0.005). The area under the receiver operating characteristic curve (AUC) of miR-486-5p at 0-6 h of age to differentiate the perinatal asphyxia group from the healthy control group was 0.831, and the AUC to differentiate newborns eligible for therapeutic hypothermia from others was 0.782. In addition, a lower expression of miR-486-5p at 7 days of age was noted in the asphyxiated newborns with adverse outcomes compared to those with normal outcomes.

Conclusion: MiR-486-5p may be a biomarker of perinatal asphyxia in newborns, and further research is warranted to clarify its role.

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循环微RNA是围产期窒息的生物标记特征。

目的：本研究旨在探讨微RNA（miRNA）是否可作为围产期窒息的生物标志物，以及它们是否与脑磁共振成像的严重程度相关：我们对 26 名足月新生儿进行了前瞻性研究，其中包括 10 名围产期窒息新生儿和 16 名健康对照组新生儿。在新生儿出生后 0-6 小时和 7 天采集血浆样本。根据修改后的萨纳特分期对脑病进行分类。对出生后 30 天内的存活婴儿进行了磁共振成像，并确定了评分。我们使用新一代测序技术研究了不同表达的 miRNA，然后使用定量反转录实时聚合酶链反应（RT-PCR）对其进行了进一步验证：结果：与健康对照组相比，窒息新生儿在出生后 0-6 h 的 miR-486-5p 表达量明显较低（p = 0.005）。用于区分围产期窒息组和健康对照组的 0-6 h miR-486-5p 接收操作特征曲线下面积（AUC）为 0.831，用于区分符合治疗性低温条件的新生儿和其他新生儿的接收操作特征曲线下面积（AUC）为 0.782。此外，与预后正常的新生儿相比，预后不良的窒息新生儿在出生7天时的miR-486-5p表达量较低：结论：MiR-486-5p可能是新生儿围产期窒息的生物标志物，需要进一步研究以明确其作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pediatrics and Neonatology PEDIATRICS-

CiteScore

3.10

自引率

0.00%

发文量

170

审稿时长

48 days

期刊介绍： Pediatrics and Neonatology is the official peer-reviewed publication of the Taiwan Pediatric Association and The Society of Neonatology ROC, and is indexed in EMBASE and SCOPUS. Articles on clinical and laboratory research in pediatrics and related fields are eligible for consideration.