Molecular and serological screening of coxiellosis in bovines with reproductive disorders in dairy farms

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Neha Parmar , Satyaveer Singh Malik , Manesh Kumar , Jay Prakash Yadav , V.K. Vinod , M.S. Sivaprasad , Brijesh Kumar , Jess Vergis , E.S. Sanjumon , Sukhadeo Baliram Barbuddhe , Deepak Bhiwa Rawool
{"title":"Molecular and serological screening of coxiellosis in bovines with reproductive disorders in dairy farms","authors":"Neha Parmar ,&nbsp;Satyaveer Singh Malik ,&nbsp;Manesh Kumar ,&nbsp;Jay Prakash Yadav ,&nbsp;V.K. Vinod ,&nbsp;M.S. Sivaprasad ,&nbsp;Brijesh Kumar ,&nbsp;Jess Vergis ,&nbsp;E.S. Sanjumon ,&nbsp;Sukhadeo Baliram Barbuddhe ,&nbsp;Deepak Bhiwa Rawool","doi":"10.1016/j.tvjl.2024.106229","DOIUrl":null,"url":null,"abstract":"<div><p><em>Coxiella burnetii</em> infection is an emerging/re-emerging public health problem affecting several countries worldwide. In India, the disease is mainly underdiagnosed, creating hindrances in its effective control. This study investigated the occurrence of <em>C. burnetii</em> among apparently healthy cattle and cattle with a history of reproductive disorders by both PCR and indirect-ELISA. A total of 731 clinical samples (serum: 531, and vaginal swabs as well as blood: 100 each) from 531 cattle were screened for coxiellosis. The serum, blood, and vaginal swabs each collected from 100 cattle with a history of reproductive disorders were screened using Com1-PCR, Trans-PCR, and indirect-ELISA. Conversely, serum samples obtained from apparently healthy cattle were exclusively screened using indirect ELISA. None of the samples tested could detect <em>C. burnetii</em> in PCR assays, while 13.37 % of serum samples were found to be seropositive in i-ELISA. Seropositivity noted among clinically healthy and those suffering from reproductive disorders were 12.76 % and 16 %, respectively, exhibiting a non-significant difference observed between these two categories. The obtained results suggested that the occurrence of coxiellosis did not differ significantly between clinically healthy animals and those with reproductive disorders; hence, in farms affected with <em>C. burnetii</em> infection, screening healthy and symptomatic animals is crucial to implement appropriate preventive measures.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary journal","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1090023324001680","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Coxiella burnetii infection is an emerging/re-emerging public health problem affecting several countries worldwide. In India, the disease is mainly underdiagnosed, creating hindrances in its effective control. This study investigated the occurrence of C. burnetii among apparently healthy cattle and cattle with a history of reproductive disorders by both PCR and indirect-ELISA. A total of 731 clinical samples (serum: 531, and vaginal swabs as well as blood: 100 each) from 531 cattle were screened for coxiellosis. The serum, blood, and vaginal swabs each collected from 100 cattle with a history of reproductive disorders were screened using Com1-PCR, Trans-PCR, and indirect-ELISA. Conversely, serum samples obtained from apparently healthy cattle were exclusively screened using indirect ELISA. None of the samples tested could detect C. burnetii in PCR assays, while 13.37 % of serum samples were found to be seropositive in i-ELISA. Seropositivity noted among clinically healthy and those suffering from reproductive disorders were 12.76 % and 16 %, respectively, exhibiting a non-significant difference observed between these two categories. The obtained results suggested that the occurrence of coxiellosis did not differ significantly between clinically healthy animals and those with reproductive disorders; hence, in farms affected with C. burnetii infection, screening healthy and symptomatic animals is crucial to implement appropriate preventive measures.

对奶牛场中患有繁殖障碍的牛群进行柯萨氏杆菌病的分子和血清学筛查。
烧伤柯西氏菌感染是一个新出现/再次出现的公共卫生问题,影响着全球多个国家。在印度,这种疾病主要诊断不足,妨碍了有效控制。本研究通过聚合酶链式反应(PCR)和间接酶联免疫吸附试验(ELISA)调查了明显健康的牛和有生殖障碍病史的牛中烧伤弧菌的发生情况。共对 531 头牛的 731 份临床样本(血清 531 份、阴道拭子和血液各 100 份)进行了柯西氏菌病筛查。使用 Com1-PCR、Trans-PCR 和间接-ELISA 对从 100 头有生殖障碍病史的牛身上采集的血清、血液和阴道拭子进行了筛查。相反,从明显健康的牛身上采集的血清样本则完全采用间接 ELISA 方法进行筛查。在 PCR 检测中,没有一个样本能检测到烧伤蜱,而在 i-ELISA 检测中,发现 13.37% 的血清样本呈血清阳性。临床健康人和生殖系统疾病患者的血清阳性率分别为 12.76% 和 16%,两类人群之间的差异并不显著。结果表明,临床健康动物和繁殖障碍动物之间的柯西氏菌病发生率并无显著差异;因此,在受柏氏菌感染影响的农场中,筛查健康和有症状的动物对于采取适当的预防措施至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Veterinary journal
Veterinary journal 农林科学-兽医学
CiteScore
4.10
自引率
4.50%
发文量
79
审稿时长
40 days
期刊介绍: The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信