Alzheimer's Disease-Related Analytes Amyloid-β and Tau in Perilymph: Correlation With Patient Age and Cognitive Score.

IF 2.6 3区 医学 Q1 OTORHINOLARYNGOLOGY
Otolaryngology- Head and Neck Surgery Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI:10.1002/ohn.942
Amit Walia, Matthew A Shew, Nedim Durakovic, Jacques A Herzog, John R Cirrito, Carla M Yuede, Cameron C Wick, Melissa Manis, David M Holtzman, Craig A Buchman, Mark A Rutherford
{"title":"Alzheimer's Disease-Related Analytes Amyloid-β and Tau in Perilymph: Correlation With Patient Age and Cognitive Score.","authors":"Amit Walia, Matthew A Shew, Nedim Durakovic, Jacques A Herzog, John R Cirrito, Carla M Yuede, Cameron C Wick, Melissa Manis, David M Holtzman, Craig A Buchman, Mark A Rutherford","doi":"10.1002/ohn.942","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To describe the collection methods for perilymph fluid biopsy during cochlear implantation, detect levels of amyloid β 42 and 40 (Aβ<sub>42</sub> and Aβ<sub>40</sub>), and total tau (tTau) analytes with a high-precision assay, to compare these levels with patient age and Montreal Cognitive Assessment (MoCA) scores, and explore potential mechanisms and relationships with otic pathology.</p><p><strong>Study design: </strong>Prospective study.</p><p><strong>Setting: </strong>Tertiary referral center.</p><p><strong>Methods: </strong>Perilymph was collected from 25 patients using polyimide tubing to avoid amyloid adherence to glass, and analyzed with a single-molecule array advanced digital enzyme-linked immunosorbent assay platform for Aβ<sub>40</sub>, Aβ<sub>42</sub>, and tTau. Cognition was assessed by MoCA.</p><p><strong>Results: </strong>Perilymph volumes ranged from ∼1 to 13 µL, with analyte concentrations spanning 2.67 to 1088.26 pg/mL. All samples had detectable levels of tTau, Aβ<sub>40</sub>, and Aβ<sub>42</sub>, with a significant positive correlation between Aβ<sub>42</sub> and Aβ<sub>40</sub> levels. Levels of Aβ<sub>42</sub>, Aβ<sub>40</sub>, and tTau were positively correlated with age, while MoCA scores were inversely correlated with age. tTau and Aβ<sub>42</sub>/Aβ<sub>40</sub>-ratios were significantly correlated with MoCA scores.</p><p><strong>Conclusion: </strong>Alzheimer's disease-associated peptides Aβ<sub>42</sub>, Aβ<sub>40</sub>, and tau analytes are detectable in human perilymph at levels approximately 10-fold lower than those found in cerebrospinal fluid (CSF). These species increase with age and correlate with cognitive impairment indicators, suggesting their potential utility as biomarkers for cognitive impairment in patients undergoing cochlear implantation. Future research should investigate the origin of these analytes in the perilymph and their potential links to inner ear pathologies and hearing loss, as well as their relationships to CSF and plasma levels in individuals.</p>","PeriodicalId":19707,"journal":{"name":"Otolaryngology- Head and Neck Surgery","volume":" ","pages":"1850-1858"},"PeriodicalIF":2.6000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11606756/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Otolaryngology- Head and Neck Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/ohn.942","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/27 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: To describe the collection methods for perilymph fluid biopsy during cochlear implantation, detect levels of amyloid β 42 and 40 (Aβ42 and Aβ40), and total tau (tTau) analytes with a high-precision assay, to compare these levels with patient age and Montreal Cognitive Assessment (MoCA) scores, and explore potential mechanisms and relationships with otic pathology.

Study design: Prospective study.

Setting: Tertiary referral center.

Methods: Perilymph was collected from 25 patients using polyimide tubing to avoid amyloid adherence to glass, and analyzed with a single-molecule array advanced digital enzyme-linked immunosorbent assay platform for Aβ40, Aβ42, and tTau. Cognition was assessed by MoCA.

Results: Perilymph volumes ranged from ∼1 to 13 µL, with analyte concentrations spanning 2.67 to 1088.26 pg/mL. All samples had detectable levels of tTau, Aβ40, and Aβ42, with a significant positive correlation between Aβ42 and Aβ40 levels. Levels of Aβ42, Aβ40, and tTau were positively correlated with age, while MoCA scores were inversely correlated with age. tTau and Aβ42/Aβ40-ratios were significantly correlated with MoCA scores.

Conclusion: Alzheimer's disease-associated peptides Aβ42, Aβ40, and tau analytes are detectable in human perilymph at levels approximately 10-fold lower than those found in cerebrospinal fluid (CSF). These species increase with age and correlate with cognitive impairment indicators, suggesting their potential utility as biomarkers for cognitive impairment in patients undergoing cochlear implantation. Future research should investigate the origin of these analytes in the perilymph and their potential links to inner ear pathologies and hearing loss, as well as their relationships to CSF and plasma levels in individuals.

老年痴呆症相关分析物淀粉样蛋白-β和脑周淋巴细胞中的 Tau:与患者年龄和认知评分的相关性
目的描述人工耳蜗植入术中淋巴结活检的收集方法,用高精度测定法检测淀粉样β 42和40(Aβ42和Aβ40)以及总tau(tTau)分析物的水平,比较这些水平与患者年龄和蒙特利尔认知评估(MoCA)评分的关系,并探讨与耳病理学的潜在机制和关系:研究设计:前瞻性研究:研究设计:前瞻性研究:使用聚酰亚胺管收集25名患者的耳道分泌物,以避免淀粉样蛋白附着在玻璃上,并使用单分子阵列高级数字酶联免疫吸附测定平台分析Aβ40、Aβ42和tTau。认知能力通过MoCA进行评估:虹膜周体积从1微升到13微升不等,分析物浓度从2.67皮克/毫升到1088.26皮克/毫升不等。所有样本都能检测到 tTau、Aβ40 和 Aβ42,其中 Aβ42 和 Aβ40 的水平呈显著正相关。Aβ42、Aβ40和tTau水平与年龄呈正相关,而MoCA评分与年龄成反比:结论:阿尔茨海默病相关肽 Aβ42、Aβ40 和 tau 分析物可在人耳周液中检测到,其水平比脑脊液(CSF)中低约 10 倍。这些物质会随着年龄的增长而增加,并与认知障碍指标相关,这表明它们有可能成为人工耳蜗植入患者认知障碍的生物标志物。未来的研究应调查这些分析物在耳周液中的来源及其与内耳病变和听力损失的潜在联系,以及它们与个体脑脊液和血浆水平的关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Otolaryngology- Head and Neck Surgery
Otolaryngology- Head and Neck Surgery 医学-耳鼻喉科学
CiteScore
6.70
自引率
2.90%
发文量
250
审稿时长
2-4 weeks
期刊介绍: Otolaryngology–Head and Neck Surgery (OTO-HNS) is the official peer-reviewed publication of the American Academy of Otolaryngology–Head and Neck Surgery Foundation. The mission of Otolaryngology–Head and Neck Surgery is to publish contemporary, ethical, clinically relevant information in otolaryngology, head and neck surgery (ear, nose, throat, head, and neck disorders) that can be used by otolaryngologists, clinicians, scientists, and specialists to improve patient care and public health.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信