Determination of amino acids and peptides without their pre-column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview
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引用次数: 0
Abstract
This review provides an overview of recent works focusing on the determination of amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection and ultraviolet (UV) detection, which is the most widespread detection in capillary electromigration techniques, without pre-capillary derivatization. Available options for the UV detection of these analytes, such as indirect detection, complexation with transition metal ions, and in-capillary derivatization are described. Developments in the field of direct detection of UV-absorbing AAs and peptides as well as progress in chiral separation are described. A separate section is dedicated to using on-line sample preconcentration methods combined with capillary electrophoresis-UV.
本综述概述了近期的研究工作，重点是利用毛细管电泳的非接触式电导检测和紫外（UV）检测（这是毛细管电迁移技术中最常用的检测方法）测定氨基酸（AA）和肽，而无需预先进行毛细管衍生。本文介绍了紫外检测这些分析物的可用方案，如间接检测、与过渡金属离子络合以及毛细管内衍生。此外，还介绍了紫外吸收 AA 和肽直接检测领域的发展以及手性分离方面的进展。另有一节专门介绍与毛细管电泳-紫外结合使用的在线样品预浓缩方法。
期刊介绍：
The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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