An automated faecal egg count system for detection of Ascaridia galli ova in chickens.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
J L Cain, D Wilson, P Slusarewicz
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引用次数: 0

Abstract

Chicken production has increased over the past decade, resulting in a concomitant rise in the demand for more humane options for poultry products including cage-free, free-range, and organic meat and eggs. These husbandry changes, however, have come hand-in-hand with increased prevalence of Ascaridia galli infection, which can cause clinical disease in chickens as well as the occasional appearance of worms in eggs. Additionally, development of anthelmintic resistance in closely related helminths of turkeys highlights the need for closely monitored anthelmintic treatment programs. Manual faecal egg counts (FECs) can be time-consuming and require specialist training. As such, this study sought to validate an automated FEC system for use in detection and quantification of A. galli eggs in chicken faeces. Automated counts using the Parasight System (PS) were compared to traditional manual McMaster counting for both precision and correlation between methods. Overall, ten repeated counts were performed on twenty individual samples for a total of 200 counts performed for each method. A strong, statistically significant correlation was found between methods (R2 = 0.7879, P < 0.0001), and PS counted more eggs and performed with statistically significant higher precision (P = 0.0391) than manual McMaster counting. This study suggests that PS is a good alternative method for performing A. galli FECs and provides a new tool for use in helminth treatment and control programs in chicken operations.

用于检测鸡五倍子蛔虫卵的自动粪蛋计数系统。
在过去的十年中,鸡的产量不断增加,因此对更人性化的家禽产品(包括无笼养、散养和有机肉蛋)的需求也随之增加。然而,在饲养方式发生变化的同时,鸡五倍子蛔虫感染的发病率也在上升,这种疾病可导致鸡的临床疾病,偶尔还会在鸡蛋中出现蠕虫。此外,与火鸡密切相关的蠕虫对驱虫药产生了抗药性,这也凸显了密切监控驱虫药治疗计划的必要性。人工粪便虫卵计数(FECs)非常耗时,而且需要专业培训。因此,本研究试图验证用于检测和定量鸡粪便中加利虫卵的自动粪便计数系统。使用 Parasight 系统 (PS) 进行的自动计数与传统的人工麦克马斯特计数进行了比较,以确定两种方法的精确度和相关性。总体而言,每种方法都对 20 个样本进行了 10 次重复计数,总共进行了 200 次计数。与人工麦克马斯特计数法相比,PS计数法计数的卵数更多,精度更高(P = 0.0391)。这项研究表明,PS 是进行 A. galli FECs 的一种很好的替代方法,为养鸡场的蠕虫治疗和控制项目提供了一种新工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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