Effects of postbiotics on chronic diarrhea in young adults: a randomized, double-blind, placebo-controlled crossover trial assessing clinical symptoms, gut microbiota, and metabolite profiles.

IF 12.2 1区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Gut Microbes Pub Date : 2024-01-01 Epub Date: 2024-08-27 DOI:10.1080/19490976.2024.2395092
Shuai Guo, Teng Ma, Lai-Yu Kwok, Keyu Quan, Bohai Li, Huan Wang, Heping Zhang, Bilige Menghe, Yongfu Chen
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Abstract

Chronic diarrhea has a considerable impact on quality of life. This randomized, double-blind, placebo-controlled crossover intervention trial was conducted with 69 participants (36 in Group A, 33 in Group B), aiming to investigate the potential of postbiotics in alleviating diarrhea-associated symptoms. Participants received postbiotic Probio-Eco® and placebo for 21 days each in alternating order, with a 14-day washout period between interventions. The results showed that postbiotic intake resulted in significant improvements in Bristol stool scale score, defecation frequency, urgency, and anxiety. Moreover, the postbiotic intervention increased beneficial intestinal bacteria, including Dysosmobacter welbionis and Faecalibacterium prausnitzii, while reducing potential pathogens like Megamonas funiformis. The levels of gut Microviridae notably increased. Non-targeted metabolomics analysis revealed postbiotic-driven enrichment of beneficial metabolites, including α-linolenic acid and p-methoxycinnamic acid, and reduction of diarrhea-associated metabolites, including theophylline, piperine, capsaicin, and phenylalanine. Targeted metabolomics confirmed a significant increase in fecal butyric acid after postbiotic intervention. The levels of aromatic amino acids, phenylalanine and tryptophan, and their related metabolites, 5-hydroxytryptophan and kynurenine, decreased after the postbiotic intervention, suggesting diarrhea alleviation was through modulating the tryptophan-5-hydroxytryptamine and tryptophan-kynurenine pathways. Additionally, chenodeoxycholic acid, a diarrhea-linked primary bile acid, decreased substantially. In conclusion, postbiotics have shown promise in relieving chronic diarrhea.

益生菌后对年轻人慢性腹泻的影响:一项评估临床症状、肠道微生物群和代谢物特征的随机、双盲、安慰剂对照交叉试验。
慢性腹泻对生活质量有很大影响。这项随机、双盲、安慰剂对照交叉干预试验有 69 名参与者参加(A 组 36 人,B 组 33 人),旨在研究益生菌在缓解腹泻相关症状方面的潜力。参与者交替服用益生菌 Probio-Eco® 和安慰剂各 21 天,两次干预之间有 14 天的冲洗期。结果显示,摄入益生菌后,布里斯托粪便量表评分、排便频率、紧迫感和焦虑感都有明显改善。此外,后益生菌干预增加了肠道有益菌,包括韦尔比奥尼氏痢疾杆菌和普鲁斯尼茨氏粪杆菌,同时减少了潜在的病原体,如痢疾杆菌。肠道微小病毒科的数量明显增加。非靶向代谢组学分析表明,后益生菌驱动富集了有益代谢物,包括α-亚麻酸和对甲氧基肉桂酸,减少了与腹泻有关的代谢物,包括茶碱、胡椒碱、辣椒碱和苯丙氨酸。靶向代谢组学证实,益生菌干预后,粪便中的丁酸含量显著增加。芳香族氨基酸、苯丙氨酸和色氨酸及其相关代谢物 5- 羟色氨酸和犬尿氨酸的水平在后益生菌干预后有所下降,这表明腹泻的缓解是通过调节色氨酸-5-羟色胺和色氨酸-犬尿氨酸途径实现的。此外,与腹泻相关的主要胆汁酸--酚去氧胆酸也大幅减少。总之,益生菌后药物在缓解慢性腹泻方面大有可为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Gut Microbes
Gut Microbes Medicine-Microbiology (medical)
CiteScore
18.20
自引率
3.30%
发文量
196
审稿时长
10 weeks
期刊介绍: The intestinal microbiota plays a crucial role in human physiology, influencing various aspects of health and disease such as nutrition, obesity, brain function, allergic responses, immunity, inflammatory bowel disease, irritable bowel syndrome, cancer development, cardiac disease, liver disease, and more. Gut Microbes serves as a platform for showcasing and discussing state-of-the-art research related to the microorganisms present in the intestine. The journal emphasizes mechanistic and cause-and-effect studies. Additionally, it has a counterpart, Gut Microbes Reports, which places a greater focus on emerging topics and comparative and incremental studies.
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