Comparative analysis of Escherichia coli Nissle 1917 ghosts quality: a study of two chemical methods

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-08-27 DOI:10.1007/s00203-024-04095-0

Fahimeh Salasar Moghaddam, Mobina Tabibian, Moloud Absalan, Gholamreza Tavoosidana, Mohammad Hossein Ghahremani, Nasrollah Tabatabaei, Mansoreh Abdolhosseini, Mahdi Shafiee Sabet, Elahe Motevaseli

{"title":"Comparative analysis of Escherichia coli Nissle 1917 ghosts quality: a study of two chemical methods","authors":"Fahimeh Salasar Moghaddam, Mobina Tabibian, Moloud Absalan, Gholamreza Tavoosidana, Mohammad Hossein Ghahremani, Nasrollah Tabatabaei, Mansoreh Abdolhosseini, Mahdi Shafiee Sabet, Elahe Motevaseli","doi":"10.1007/s00203-024-04095-0","DOIUrl":null,"url":null,"abstract":"<div><p>The gram-negative bacterium <i>Escherichia coli</i> Nissle 1917 (EcN) has long been recognized for its therapeutic potential in treating various intestinal diseases. Bacterial ghosts (BGs) are empty shells of non-living bacterial cells that demonstrate enormous potential for medicinal applications. Genetic and chemical techniques can create these BGs. In the current study, we produced <i>Escherichia coli</i> Nissle 1917 ghosts (EcNGs) for the first time using benzoic acid (BA) and sodium hydroxide (SH). BA is a feeble acidic chemical that enhances gram-negative bacteria’s external membrane permeability, reduces energy production, and decreases internal pH. SH has shown success in producing BGs from some gram-negative and gram-positive organisms. This research aims to produce EcNGs using the minimum inhibitory concentration (MIC) of SH and BA, specifically 3.125 mg/mL. We assessed the bacterial quality of the BGs produced using quantitative PCR (qPCR) and Bradford protein assays. Field emission scanning electron microscopy (FE-SEM) showed the three-dimensional structure of EcNGs. The study confirmed the presence of tunnel-like pores on the outer surface, indicating the preservation of cell membrane integrity. Importantly, this investigation introduces BA as a novel chemical inducer of EcNGs, suggesting its potential alongside SH for efficient EcNG formation.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00203-024-04095-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The gram-negative bacterium Escherichia coli Nissle 1917 (EcN) has long been recognized for its therapeutic potential in treating various intestinal diseases. Bacterial ghosts (BGs) are empty shells of non-living bacterial cells that demonstrate enormous potential for medicinal applications. Genetic and chemical techniques can create these BGs. In the current study, we produced Escherichia coli Nissle 1917 ghosts (EcNGs) for the first time using benzoic acid (BA) and sodium hydroxide (SH). BA is a feeble acidic chemical that enhances gram-negative bacteria’s external membrane permeability, reduces energy production, and decreases internal pH. SH has shown success in producing BGs from some gram-negative and gram-positive organisms. This research aims to produce EcNGs using the minimum inhibitory concentration (MIC) of SH and BA, specifically 3.125 mg/mL. We assessed the bacterial quality of the BGs produced using quantitative PCR (qPCR) and Bradford protein assays. Field emission scanning electron microscopy (FE-SEM) showed the three-dimensional structure of EcNGs. The study confirmed the presence of tunnel-like pores on the outer surface, indicating the preservation of cell membrane integrity. Importantly, this investigation introduces BA as a novel chemical inducer of EcNGs, suggesting its potential alongside SH for efficient EcNG formation.

Graphical Abstract

Abstract Image

查看原文本刊更多论文

大肠杆菌 Nissle 1917 幽灵质量的比较分析：两种化学方法的研究。

革兰氏阴性大肠杆菌 Nissle 1917（EcN）在治疗各种肠道疾病方面的治疗潜力早已得到公认。细菌幽灵（BGs）是无生命细菌细胞的空壳，具有巨大的医药应用潜力。遗传和化学技术可以制造这些 BGs。在本研究中，我们首次使用苯甲酸（BA）和氢氧化钠（SH）制造了大肠杆菌尼氏 1917 幽灵（EcNGs）。苯甲酸是一种弱酸性化学物质，可增强革兰氏阴性细菌的外膜渗透性、减少能量产生并降低内部 pH 值。SH 已成功地从一些革兰氏阴性和革兰氏阳性菌中生产出 BGs。本研究旨在利用 SH 和 BA 的最小抑菌浓度（MIC）（具体为 3.125 毫克/毫升）来生产 EcNG。我们使用定量 PCR（qPCR）和 Bradford 蛋白检测法评估了所生产的 BGs 的细菌质量。场发射扫描电子显微镜（FE-SEM）显示了 EcNGs 的三维结构。研究证实，外表面存在隧道状孔隙，表明细胞膜保持完整。重要的是，这项研究引入了 BA 作为 EcNGs 的新型化学诱导剂，表明 BA 有潜力与 SH 共同促进 EcNG 的有效形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.