Deciphering microbial communities involved in marine steel corrosion using high-throughput amplicon sequencing

IF 3.6 4区 生物学 Q2 ENVIRONMENTAL SCIENCES
Biji Shibulal, Martin Peter Smith, Ian Cooper, Heidi Marie Burgess, Norman Moles, Alison Willows
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Abstract

To characterize the source and effects of bacterial communities on corrosion of intertidal structures, three different UK coastal sites were sampled for corrosion materials, sediment and seawater. Chemical analyses indicate the activity of sulfate-reducing microbes (SRBs) at 2 sites (Shoreham and Newhaven), but not at the third (Southend-on-Sea). Microbial communities in the deep sediment and corrosion samples are similar. The phylum Proteobacteria is dominant (40.4% of the total ASV), followed by Campilobacterota (11.3%), Desulfobacterota and Firmicutes (4%–5%). At lower taxonomic levels, corrosion causing bacteria, such as Shewanella sp. (6%), Colwellia sp. (7%) and Mariprofundus sp. (1%), are present. At Southend-on-sea, the relative abundance of Campilobacterota is higher compared to the other two sites. The mechanism of action of microorganisms at Shoreham and Newhaven involves biogenic sulfuric acid corrosion of iron by the combined action of SRBs and sulfur-oxidizing microbes. However, at Southend-on-sea, sulfur compounds are not implicated in corrosion, but SRBs and other electroactive microbes may play a role in which cathodic reactions (electrical MIC) and microbial enzymes (chemical MIC) are involved. To contribute to diagnosis of accelerated intertidal corrosion types, we developed a rapid identification method for SRBs using quantitative polymerase chain reaction high-resolution melt curve analysis of the dsrB gene.

Abstract Image

Abstract Image

利用高通量扩增片段测序技术解密海洋钢腐蚀过程中的微生物群落。
为了确定细菌群落对潮间带结构腐蚀的来源和影响,对英国三个不同的沿海地点的腐蚀 材料、沉积物和海水进行了取样。化学分析结果表明,在两个地点(肖勒姆和纽黑文)有硫酸盐还原微生物 (SRB) 的活动,但在第三个地点(海边苏特)没有。深层沉积物和腐蚀样本中的微生物群落相似。变形菌门占主导地位(占 ASV 总量的 40.4%),其次是弯曲菌门(11.3%)、脱硫菌门和固着菌门(4%-5%)。在较低的分类水平上,还存在导致腐蚀的细菌,如 Shewanella sp.(6%)、Colwellia sp.(7%)和 Mariprofundus sp.(1%)。与其他两个地点相比,苏特海滨的弯曲菌群相对较多。肖勒姆和纽黑文的微生物作用机理是,在 SRB 和硫氧化微生物的共同作用下,生物硫酸腐蚀铁。不过,在海滨苏特,硫化合物与腐蚀无关,但 SRB 和其他电活性微生物可能在阴极反应(电性 MIC)和微生物酶(化学 MIC)的作用下发挥作用。为了帮助诊断加速潮间带腐蚀类型,我们利用定量聚合酶链式反应高分辨率dsrB基因熔融曲线分析,开发了一种快速鉴定SRB的方法。
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来源期刊
Environmental Microbiology Reports
Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.00
自引率
3.00%
发文量
91
审稿时长
3.0 months
期刊介绍: The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
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