Optimal environmental sampling conditions for electrostatic aerosol-to-hydrosol collection of airborne viruses.

Journal of hazardous materials Pub Date : 2024-10-05 Epub Date: 2024-08-11 DOI:10.1016/j.jhazmat.2024.135491
Amin Piri, Milad Massoudifarid, Jungho Hwang
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Abstract

Due to adverse effects of viral outbreaks on human health, accurate detection of airborne pathogens is essential. Among many methods available for bioaerosol sampling, electrostatic precipitation (ESP) has been used to directly collect bioaerosols as hydrosols. The performance of an ESP sampler depends on its design, operational and environmental parameters such as air relative humidity (RH), air temperature, sampling liquid type and liquid temperature. Thus, it is essential to identify and maintain optimal conditions throughout sampling process to operate the sampler at its highest capacity. This study provides crucial insights into parameters that affect the collection efficiency of the aerosol-to-hydrosol ESP sampler and its virus recovery. The results indicate that air temperature does not affect collection efficiency, meanwhile, air RH, sampling liquid temperature, and salt concentration are the main parameters that significantly affect collection efficiency. Likewise, when deionized water is used as sampling liquid, hydrogen peroxide concentration increases proportionally with increasing air RH, resulting in significant decrease of virus viability. Consequently, for ESP samplers similar to our study, the following conditions are recommended: air RH of 55-65%, air and sampling liquid temperature of 37 °C, and a mixture of 10-20 mM ascorbic acid in PBS as sampling liquid.

静电气溶胶-水溶胶采集空气传播病毒的最佳环境采样条件。
由于病毒爆发会对人类健康造成不利影响,因此准确检测空气中的病原体至关重要。在生物气溶胶采样的众多方法中,静电沉淀(ESP)被用来直接收集水溶胶形式的生物气溶胶。ESP 采样器的性能取决于其设计、操作和环境参数,如空气相对湿度 (RH)、空气温度、采样液体类型和液体温度。因此,必须在整个采样过程中确定并保持最佳条件,使采样器以最高能力运行。本研究对影响气溶胶-水溶胶 ESP 采样器收集效率及其病毒回收率的参数提供了重要的见解。结果表明,空气温度不会影响收集效率,而空气相对湿度、采样液温度和盐浓度则是显著影响收集效率的主要参数。同样,当使用去离子水作为采样液时,过氧化氢浓度会随着空气相对湿度的增加而成正比增加,从而导致病毒存活率显著下降。因此,对于与我们的研究类似的 ESP 采样器,建议采用以下条件:空气相对湿度为 55-65%,空气和采样液温度为 37 °C,采样液为 10-20 mM 抗坏血酸在 PBS 中的混合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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