A MYB family transcription factor TdRCA1 from wild emmer wheat regulates anthocyanin biosynthesis in coleoptile.

IF 4.4 1区 农林科学 Q1 AGRONOMY
Jinlong Li, Chaozhong Zhang, Xiangru Xu, Yuqing Su, Yutian Gao, Jiatian Yang, Chaojie Xie, Jun Ma
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Abstract

As important secondary metabolites in plants, anthocyanins not only contribute to colored plants organs, but also provide protections against various biotic and abiotic stresses. In this study, a MYB transcription factor gene TdRCA1 from wild emmer wheat regulating anthocyanin biosynthesis in wheat coleoptile was identified on the short arm of chromosome 7A in common wheat genetic background. The TdRCA1 overexpression lines showed colored callus, coleoptile, auricle and stem nodes, as well as up regulation of six anthocyanin-related structural genes. The expression of TdRCA1 was activated by light in a temporal manner. While coleoptile color of 48 and 60 h dark-grown seedlings changed from green to red after 24 h light treatment, those grown in dark for 72 and 96 h failed to develop red coleoptiles after light restoration. Interestingly, the over expression of TdRCA1 resulted in increased resistance to Fusarium crown rot, a chronic and severe fungal disease in many cereal growing regions in the world. Our results offer a better understanding of the molecular basis of coleoptile color in bread wheat.

Abstract Image

野生珙桐小麦中的 MYB 家族转录因子 TdRCA1 可调控小麦叶柄中花青素的生物合成。
花青素是植物中重要的次生代谢产物,不仅能使植物器官着色,还能抵御各种生物和非生物胁迫。本研究在普通小麦遗传背景的 7A 染色体短臂上发现了一个来自野生小麦的 MYB 转录因子基因 TdRCA1,该基因调控小麦胚叶花青素的生物合成。TdRCA1过表达株系的胼胝体、胚轴、叶耳和茎节均呈彩色,并上调了6个与花青素相关的结构基因。TdRCA1 的表达受光的激活具有时间性。经 24 小时光照处理后,48 小时和 60 小时黑暗生长的幼苗的叶柄颜色由绿色变为红色,而 72 小时和 96 小时黑暗生长的幼苗在光照恢复后未能长出红色叶柄。有趣的是,TdRCA1 的过度表达增强了对镰刀菌冠腐病的抗性。我们的研究结果有助于更好地理解面包小麦胚轴颜色的分子基础。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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