Detection of exogenous siRNA inside sweet corn bundle sheath cells and the RNAi dynamics in the early stage of Maize dwarf mosaic virus infection.

IF 3.4 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2024-08-01 Epub Date: 2024-08-14 DOI:10.1007/s12298-024-01500-2

Kinga Balassa, György Balassa, Asztéria Almási, Tamás Visnovitz, Szabolcs Rudnóy

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Abstract

Maize dwarf mosaic virus (MDMV) is one of the most serious viruses of sweet corn. Utilising the process of RNA interference, the exogenous introduction of small RNA molecules mimicking virus-derived small interfering RNA (siRNA) into the plant prior to infection triggers the antiviral RNA silencing effect, thereby promoting more effective antiviral protection. Hence, a treatment with MDMV-derived small RNA was applied to sweet corn plants one day before MDMV virus inoculation. ALEXA FLUOR®488 fluorophore-bound exogenous siRNA was successfully detected inside intact sweet corn cells using confocal fluorescence microscopy. Furthermore, it was demonstrated that the exogenous siRNA treatment led to a notable upregulation of the AGO1, AGO2b, AGO10b, AGO18a, DCL1, DCL3a, DCL4, RDR1, and MOP1 genes within 24 h of the treatment. Overall, exogenous siRNA treatment resulted in better virus control of infected sweet corn plants, as indicated by the lower viral RNA and coat protein levels compared to the infected group without pre-treatment.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-024-01500-2.

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甜玉米束鞘细胞内外源 siRNA 的检测及玉米矮化花叶病毒感染初期的 RNAi 动态。

玉米矮化花叶病毒（MDMV）是甜玉米最严重的病毒之一。利用 RNA 干扰过程，在感染前将模仿病毒衍生的小干扰 RNA（siRNA）的小 RNA 分子外源引入植株，可触发抗病毒 RNA 沉默效应，从而促进更有效的抗病毒保护。因此，在接种 MDMV 病毒前一天，对甜玉米植株施用 MDMV 衍生的小 RNA 进行处理。利用共聚焦荧光显微镜，成功地在完整的甜玉米细胞内检测到了与 ALEXA FLUOR®488 荧光团结合的外源 siRNA。此外，研究表明，外源 siRNA 处理后 24 小时内，AGO1、AGO2b、AGO10b、AGO18a、DCL1、DCL3a、DCL4、RDR1 和 MOP1 基因明显上调。总体而言，外源 siRNA 处理能更好地控制感染甜玉米植株的病毒，与未进行预处理的感染组相比，病毒 RNA 和衣壳蛋白水平较低：在线版本包含补充材料，可查阅 10.1007/s12298-024-01500-2。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.