Sensitivity Variations in Developmental Toxicity of Imidacloprid to Zebrafish Embryos at Different Neurodevelopmental Stages

IF 3.6 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental Toxicology and Chemistry Pub Date : 2024-08-26 DOI:10.1002/etc.5986

Zewei Xu, Lingzhi Xie, Huizhen Li, Jing You

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Abstract

Neonicotinoids are ubiquitous in global surface waters and pose a significant risk to aquatic organisms. However, information is lacking on the variations in sensitivity of organisms at different developmental stages to the neurotoxic neonicotinoids. We established a spectrum of toxicity to zebrafish embryos at four neurodevelopmental stages (1, 3, 6, and 8 h post fertilization [hpf]) and dechorionated embryos at 6 hpf based on external and internal exposure to imidacloprid as a representative neonicotinoid. Embryos at the gastrula stage (6 and 8 hpf) were more sensitive to imidacloprid than embryos at earlier developmental stages. Dechorionated embryos were more sensitive to imidacloprid than embryos with a chorion, suggesting that the chorion offers protection against pollutants. Nine sublethal effects were induced by imidacloprid exposure, among which uninflated swim bladder (USB) was the most sensitive. Water depth and air availability in the exposure chambers were critical factors influencing the occurrence of USB in zebrafish larvae. Internal residues of metabolites accounted for <10% of imidacloprid, indicating that imidacloprid was metabolized in a limited fashion in the embryos. In addition, acute toxicity of the main metabolite 5-hydroxy-imidacloprid was significantly lower than that of imidacloprid, indicating that the observed toxicity in embryos exposed to imidacloprid was mainly induced by the parent compound. Our research offers a fresh perspective on choosing the initial exposure time in zebrafish embryo toxicity tests, particularly for neurotoxicants. Environ Toxicol Chem 2024;43:2398–2408. © 2024 SETAC

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不同神经发育阶段斑马鱼胚胎对吡虫啉发育毒性的敏感性差异

新烟碱类化合物在全球地表水中无处不在，对水生生物构成了重大风险。然而，关于不同发育阶段的生物对具有神经毒性的新烟碱类物质的敏感性差异，目前还缺乏相关信息。我们根据对吡虫啉（一种具有代表性的新烟碱类杀虫剂）的外部和内部接触，建立了斑马鱼胚胎在四个神经发育阶段（受精后 1、3、6 和 8 hpf）以及脱绒胚胎在 6 hpf 的毒性谱系。与较早发育阶段的胚胎相比，处于胚盘期（6 和 8 hpf）的胚胎对吡虫啉更为敏感。与有绒毛的胚胎相比，无绒毛的胚胎对吡虫啉更敏感，这表明绒毛对污染物具有保护作用。接触吡虫啉诱发了九种亚致死效应，其中未充气鳔（USB）最为敏感。暴露室的水深和空气供应是影响斑马鱼幼体出现 USB 的关键因素。代谢物的内部残留占到

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来源期刊

Environmental Toxicology and Chemistry 环境科学-毒理学

CiteScore

7.40

自引率

9.80%

发文量

265

审稿时长

3.4 months

期刊介绍： The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.