In vitro characterisation of a novel rubber contact allergen in protective gloves

IF 4.8 1区医学 Q2 ALLERGY

Contact Dermatitis Pub Date : 2024-08-25 DOI:10.1111/cod.14682

Renato Ivan de Ávila, Linda Ljungberg Silic, Sofía Carreira-Santos, Gábor Merényi, Ola Bergendorff, Kathrin S. Zeller

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引用次数: 0

Abstract

Background

Allergic contact dermatitis (ACD) from protective gloves is often caused by rubber additives, such as accelerators. However, while accelerator-free rubber gloves are available, they still cause ACD in some individuals.

Objectives

A new allergen, 2-cyаnоethyl dimethyldithiocarbamate, (CEDMC), has recently been identified in accelerator-free gloves, and we here provide a first in vitro characterisation of CEDMC in a dendritic cell (DC)-like cell model along with three reference sensitizer rubber chemicals, consisting of tetraethylthiuram disulfide (TETD) and two xanthogenates.

Methods

Cellular responses after the exposure to the rubber chemicals were assessed using a transcriptomic approach, multiplex cytokine secretion profiling, and flow cytometry to determine DC model activation marker expression and apoptosis induction.

Results

CEDMC and all other sensitizers were classified as strong skin sensitizers with the transcriptomic approach. They all significantly increased IL-8 secretion and exposure to all except one increased CD86 DC activation marker expression. When tested, CEDMC induced apoptosis, however, delayed compared to TETD.

Conclusions

The in vitro data corroborate CEDMC, TETD, and investigated xanthogenates as skin sensitizers. Transcriptomic analyses further reveal unique cellular responses induced by CEDMC, which together with future study can contribute to better understanding of cellular mechanisms underlying the sensitising capacity of rubber chemicals.

Abstract Image

查看原文本刊更多论文

防护手套中一种新型橡胶接触过敏原的体外特性分析。

背景：防护手套引起的过敏性接触性皮炎（ACD）通常是由橡胶添加剂（如促进剂）引起的。然而，虽然现在有了不含促进剂的橡胶手套，但它们仍会导致一些人出现过敏性接触性皮炎：目的：最近在无促进剂手套中发现了一种新的过敏原--2-氰基о乙基二甲基二硫代氨基甲酸酯（CEDMC），我们在此首次在树突状细胞（DC）样细胞模型中对 CEDMC 以及三种参考致敏橡胶化学物质（包括二硫化四乙基秋兰姆（TETD）和两种黄原酸盐）进行了体外表征：方法：采用转录组学方法、多重细胞因子分泌谱分析和流式细胞术评估暴露于橡胶化学品后的细胞反应，以确定 DC 模型活化标志物的表达和凋亡诱导：结果：通过转录组学方法，CEDMC 和所有其他致敏剂都被归类为强皮肤致敏剂。它们都能明显增加 IL-8 的分泌，而且除一种外，接触其他所有致敏剂都能增加 CD86 DC 激活标记物的表达。经测试，CEDMC可诱导细胞凋亡，但与TETD相比有所延迟：体外数据证实了 CEDMC、TETD 和经研究的黄原酸盐是皮肤致敏物质。转录组分析进一步揭示了 CEDMC 所诱导的独特细胞反应，这些反应以及未来的研究将有助于更好地了解橡胶化学品致敏能力的细胞机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Contact Dermatitis 医学-过敏

CiteScore

4.60

自引率

30.90%

发文量

227

审稿时长

4-8 weeks

期刊介绍： Contact Dermatitis is designed primarily as a journal for clinicians who are interested in various aspects of environmental dermatitis. This includes both allergic and irritant (toxic) types of contact dermatitis, occupational (industrial) dermatitis and consumers" dermatitis from such products as cosmetics and toiletries. The journal aims at promoting and maintaining communication among dermatologists, industrial physicians, allergists and clinical immunologists, as well as chemists and research workers involved in industry and the production of consumer goods. Papers are invited on clinical observations, diagnosis and methods of investigation of patients, therapeutic measures, organisation and legislation relating to the control of occupational and consumers".