Regulation of cryptochrome-mediated blue light signaling by the ABI4-PIF4 module.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Pengyu Song, Zidan Yang, Huaichang Wang, Fangfang Wan, Dingming Kang, Wenming Zheng, Zhizhong Gong, Jigang Li
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引用次数: 0

Abstract

ABSCISIC ACID-INSENSITIVE 4 (ABI4) is a pivotal transcription factor which coordinates multiple aspects of plant growth and development as well as plant responses to environmental stresses. ABI4 has been shown to be involved in regulating seedling photomorphogenesis; however, the underlying mechanism remains elusive. Here, we show that the role of ABI4 in regulating photomorphogenesis is generally regulated by sucrose, but ABI4 promotes hypocotyl elongation of Arabidopsis seedlings under blue (B) light under all tested sucrose concentrations. We further show that ABI4 physically interacts with PHYTOCHROME INTERACTING FACTOR 4 (PIF4), a well-characterized growth-promoting transcription factor, and post-translationally promotes PIF4 protein accumulation under B light. Further analyses indicate that ABI4 directly interacts with the B light photoreceptors cryptochromes (CRYs) and inhibits the interactions between CRYs and PIF4, thus relieving CRY-mediated repression of PIF4 protein accumulation. In addition, while ABI4 could directly activate its own expression, CRYs enhance, whereas PIF4 inhibits, ABI4-mediated activation of the ABI4 promoter. Together, our study demonstrates that the ABI4-PIF4 module plays an important role in mediating CRY-induced B light signaling in Arabidopsis.

ABI4-PIF4 模块对隐色素介导的蓝光信号转导的调控。
乙酰丙酸无感 4(ABI4)是一个关键的转录因子,它协调植物生长发育的多个方面以及植物对环境胁迫的反应。ABI4 已被证明参与调控幼苗的光形态发生;然而,其潜在机制仍然难以捉摸。在这里,我们发现 ABI4 在调控光形态发生中的作用一般受蔗糖调控,但在所有测试的蔗糖浓度下,ABI4 都能促进拟南芥幼苗在蓝光(B)下的下胚轴伸长。我们进一步发现,ABI4 与 PHYTOCHROME INTERACTING FACTOR 4(PIF4)--一种特征明确的生长促进转录因子--发生了物理相互作用,并在翻译后促进了 PIF4 蛋白在 B 光下的积累。进一步的分析表明,ABI4 可直接与 B 光感光隐色体(CRYs)相互作用,抑制 CRYs 与 PIF4 之间的相互作用,从而缓解 CRY 介导的对 PIF4 蛋白积累的抑制。此外,虽然 ABI4 可直接激活自身的表达,但 CRYs 可增强而 PIF4 可抑制 ABI4 介导的 ABI4 启动子的激活。总之,我们的研究表明,ABI4-PIF4 模块在拟南芥中介导 CRY 诱导的 B 光信号转导中起着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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