Anchorene, a carotenoid-derived growth regulator, modulates auxin homeostasis by suppressing GH3-mediated auxin conjugation

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Danping Ke, Yinpeng Xie, Haipeng Li, Liqun Hu, Yi He, Chao Guo, Yahui Zhai, Jinggong Guo, Kun Li, Zongyan Chu, Junli Zhang, Xuebin Zhang, Salim Al-Babili, Kai Jiang, Yuchen Miao, Kun-Peng Jia
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Abstract

Anchorene, identified as an endogenous bioactive carotenoid-derived dialdehyde and diapocarotenoid, affects root development by modulating auxin homeostasis. However, the precise interaction between anchorene and auxin, as well as the mechanisms by which anchorene modulates auxin levels, remain largely elusive. In this study, we conducted a comparative analysis of anchorene's bioactivities alongside auxin and observed that anchorene induces multifaceted auxin-like effects. Through genetic and pharmacological examinations, we revealed that anchorene's auxin-like activities depend on the indole-3-pyruvate-dependent auxin biosynthesis pathway, as well as the auxin inactivation pathway mediated by Group II Gretchen Hagen 3 (GH3) proteins that mainly facilitate the conjugation of indole-3-acetic acid (IAA) to amino acids, leading to the formation of inactivated storage forms. Our measurements indicated that anchorene treatment elevates IAA levels while reducing the quantities of inactivated IAA–amino acid conjugates and oxIAA. RNA sequencing further revealed that anchorene triggers the expression of numerous auxin-responsive genes in a manner reliant on Group II GH3s. Additionally, our in vitro enzymatic assays and biolayer interferometry (BLI) assay demonstrated anchorene's robust suppression of GH3.17-mediated IAA conjugation with glutamate. Collectively, our findings highlight the significant role of carotenoid-derived metabolite anchorene in modulating auxin homeostasis, primarily through the repression of GH3-mediated IAA conjugation and inactivation pathways, offering novel insights into the regulatory mechanisms of plant bioactive apocarotenoids.

Abstract Image

Anchorene 是一种类胡萝卜素衍生的生长调节剂,它通过抑制 GH3 介导的辅助素共轭作用来调节辅助素平衡。
锚链烯是一种内源生物活性类胡萝卜素衍生二醛和二apocarotenoid,它通过调节辅素平衡来影响根系发育。然而,锚链烯与辅素之间的精确相互作用以及锚链烯调节辅素水平的机制在很大程度上仍然难以捉摸。在本研究中,我们比较分析了鲥鱼素与辅助素的生物活性,观察到鲥鱼素能诱导多方面的辅助素样效应。通过遗传学和药理学研究,我们发现锚固素的类助剂活性依赖于吲哚-3-丙酮酸依赖性助剂生物合成途径,以及由第二组格雷琴-哈根 3(GH3)蛋白介导的助剂失活途径,后者主要促进吲哚-3-乙酸(IAA)与氨基酸的共轭,从而形成失活的贮存形式。我们的测量结果表明,凤尾蕨素处理可提高IAA水平,同时减少失活的IAA-氨基酸共轭物和oxIAA的数量。RNA 测序进一步表明,凤尾蕨素以依赖于第二类 GH3s 的方式触发了大量辅助素响应基因的表达。此外,我们的体外酶学测定和生物层干涉测量法(BLI)测定表明,锚链烯能有力地抑制 GH3.17 介导的 IAA 与谷氨酸的共轭。总之,我们的研究结果凸显了类胡萝卜素衍生代谢物凤尾蕨素主要通过抑制 GH3 介导的 IAA 共轭和失活途径在调节植物生长素平衡中的重要作用,为了解植物生物活性类胡萝卜素的调控机制提供了新的视角。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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