{"title":"Off–On fluorescent benzothiazole-fused coumarin for sensitive detection of nitroreductases and hydrogen sulfide","authors":"Song Yi Yoo, Na Yoon Kim, Min Hee Lee","doi":"10.1002/bkcs.12889","DOIUrl":null,"url":null,"abstract":"<p>Hydrogen sulfide (H<sub>2</sub>S) and nitroreductases (NTR) are key biological reductants, with H<sub>2</sub>S acting as a key signaling molecule and NTR reducing nitro groups to amines in cellular metabolism. Visualizing these reducing components, often overexpressed in cancer cells or bacterial environments, is crucial for metabolic understanding. Existing fluorescent probes detect these individually, but simultaneous detection methods are unexplored. We designed probe <b>1</b>, which shows high fluorescence only when both NTR and H<sub>2</sub>S are overexpressed. The probe transformed into benzothiazole-fused coumarin by NTR, exhibiting fluorescence, and shows a stronger signal in the presence of both NTR and H<sub>2</sub>S, making it a potential tool for detecting their simultaneous overexpression.</p>","PeriodicalId":54252,"journal":{"name":"Bulletin of the Korean Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of the Korean Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bkcs.12889","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Hydrogen sulfide (H2S) and nitroreductases (NTR) are key biological reductants, with H2S acting as a key signaling molecule and NTR reducing nitro groups to amines in cellular metabolism. Visualizing these reducing components, often overexpressed in cancer cells or bacterial environments, is crucial for metabolic understanding. Existing fluorescent probes detect these individually, but simultaneous detection methods are unexplored. We designed probe 1, which shows high fluorescence only when both NTR and H2S are overexpressed. The probe transformed into benzothiazole-fused coumarin by NTR, exhibiting fluorescence, and shows a stronger signal in the presence of both NTR and H2S, making it a potential tool for detecting their simultaneous overexpression.
期刊介绍：
The Bulletin of the Korean Chemical Society is an official research journal of the Korean Chemical Society. It was founded in 1980 and reaches out to the chemical community worldwide. It is strictly peer-reviewed and welcomes Accounts, Communications, Articles, and Notes written in English. The scope of the journal covers all major areas of chemistry: analytical chemistry, electrochemistry, industrial chemistry, inorganic chemistry, life-science chemistry, macromolecular chemistry, organic synthesis, non-synthetic organic chemistry, physical chemistry, and materials chemistry.
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