Conjugated microporous polymers-scaffolded enzyme cascade systems with enhanced catalytic activity

Abstract

Enhancing catalytic activity of multi-enzyme in vitro through substrate channeling effect is promising yet challenging. Herein, conjugated microporous polymers (CMPs)-scaffolded integrated enzyme cascade systems (I-ECSs) are constructed through co-entrapping glucose oxidase (GOx) and horseradish peroxidase (HRP), in which hydrogen peroxide (H₂O₂) is the intermediate product. The interplay of low-resistance mass transfer pathway and appropriate pore wall-H₂O₂ interactions facilitates the directed transfer of H₂O₂, resulting in 2.4-fold and 5.0-fold elevation in catalytic activity compared to free ECSs and separated ECSs, respectively. The substrate channeling effect could be regulated by altering the mass ratio of GOx to HRP. Besides, I-ECSs demonstrate excellent stabilities in harsh environments and multiple recycling.