Valencene ameliorates ox-LDL induced foam cell formation by suppressing inflammation and modulating key proteins involved in the atherogenesis on THP-1 derived macrophages

IF 0.5 Q4 GENETICS & HEREDITY
Mahesh Chandran , Abhirami , Bincy Shareef , Arun Surendran , Abdul Jaleel , Janeesh Plakkal Ayyappan
{"title":"Valencene ameliorates ox-LDL induced foam cell formation by suppressing inflammation and modulating key proteins involved in the atherogenesis on THP-1 derived macrophages","authors":"Mahesh Chandran ,&nbsp;Abhirami ,&nbsp;Bincy Shareef ,&nbsp;Arun Surendran ,&nbsp;Abdul Jaleel ,&nbsp;Janeesh Plakkal Ayyappan","doi":"10.1016/j.humgen.2024.201330","DOIUrl":null,"url":null,"abstract":"<div><p>Atherosclerosis is a distinct risk factor for cardiovascular and cerebrovascular disorders, which are significant contributors to global mortality. It is defined by macrophage-derived foam cell development followed by persistent inflammation, plaque formation, fibrosis and thrombosis. Studies have shown valencene, a sesquiterpene obtained from Valencia oranges, has several health-promoting properties. However, its protective effect against atherosclerosis and foam cell models remains unexplored. The present investigation revealed that valencene treatment suppresses foam cell generation and accumulation of lipids in THP-1-derived cells macrophage models activated with oxidized low-density lipoprotein (ox-LDL), maintained in vitro. The intracellular lipid content was qualitatively and semi-quantitatively analyzed by Oil Red O staining, and the compound's cytotoxicity was assessed through the MTT assay, considering both time-dependent and dose-dependent factors. The RT-qPCR results showed promising anti-inflammatory and anti-oxidant enzyme status upon valencene treatment. The H2DCFDA staining revealed valencene's ability to reduce the oxidative stress induced by ox-LDL. Further, high-throughput proteomic profiling was carried out to identify the target proteins affected by valencene treatment and thereby explore its mechanism of action on foam cell models. Proteomic studies revealed that valencene treatment regulates the expression of several proteins associated with ox-LDL-induced inflammation, defective cholesterol homeostasis and cholesterol efflux pathways.</p></div>","PeriodicalId":29686,"journal":{"name":"Human Gene","volume":"42 ","pages":"Article 201330"},"PeriodicalIF":0.5000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Human Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2773044124000743","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

Abstract

Atherosclerosis is a distinct risk factor for cardiovascular and cerebrovascular disorders, which are significant contributors to global mortality. It is defined by macrophage-derived foam cell development followed by persistent inflammation, plaque formation, fibrosis and thrombosis. Studies have shown valencene, a sesquiterpene obtained from Valencia oranges, has several health-promoting properties. However, its protective effect against atherosclerosis and foam cell models remains unexplored. The present investigation revealed that valencene treatment suppresses foam cell generation and accumulation of lipids in THP-1-derived cells macrophage models activated with oxidized low-density lipoprotein (ox-LDL), maintained in vitro. The intracellular lipid content was qualitatively and semi-quantitatively analyzed by Oil Red O staining, and the compound's cytotoxicity was assessed through the MTT assay, considering both time-dependent and dose-dependent factors. The RT-qPCR results showed promising anti-inflammatory and anti-oxidant enzyme status upon valencene treatment. The H2DCFDA staining revealed valencene's ability to reduce the oxidative stress induced by ox-LDL. Further, high-throughput proteomic profiling was carried out to identify the target proteins affected by valencene treatment and thereby explore its mechanism of action on foam cell models. Proteomic studies revealed that valencene treatment regulates the expression of several proteins associated with ox-LDL-induced inflammation, defective cholesterol homeostasis and cholesterol efflux pathways.

Abstract Image

缬草烯通过抑制炎症和调节参与 THP-1 巨噬细胞动脉粥样硬化形成的关键蛋白,改善氧化-LDL 诱导的泡沫细胞形成
动脉粥样硬化是心脑血管疾病的一个明显风险因素,是导致全球死亡的重要因素。动脉粥样硬化的定义是由巨噬细胞衍生的泡沫细胞发展,随后出现持续性炎症、斑块形成、纤维化和血栓形成。研究表明,从瓦伦西亚橘子中提取的倍半萜类化合物缬烯烃具有多种促进健康的特性。然而,它对动脉粥样硬化和泡沫细胞模型的保护作用仍有待探索。本研究发现,缬草烯处理可抑制体外维持的经氧化低密度脂蛋白(ox-LDL)激活的 THP-1 衍生细胞巨噬细胞模型中泡沫细胞的生成和脂质的积累。通过油红 O 染色对细胞内脂质含量进行了定性和半定量分析,并通过 MTT 试验评估了该化合物的细胞毒性,同时考虑了时间依赖性和剂量依赖性因素。RT-qPCR 结果显示,缬草烯处理后具有良好的抗炎和抗氧化作用。H2DCFDA 染色显示,缬草烯能降低氧化-LDL 诱导的氧化应激。此外,还进行了高通量蛋白质组分析,以确定缬草烯处理所影响的靶蛋白,从而探索其对泡沫细胞模型的作用机制。蛋白质组学研究发现,缬草烯处理可调节与氧化-LDL诱导的炎症、胆固醇稳态缺陷和胆固醇外流途径相关的几种蛋白质的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Human Gene
Human Gene Biochemistry, Genetics and Molecular Biology (General), Genetics
CiteScore
1.60
自引率
0.00%
发文量
0
审稿时长
54 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信