Ammonium enhances rice resistance to Magnaporthe oryzae through H2O2 accumulation

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-08-19 DOI:10.1016/j.plaphy.2024.109058

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Abstract

Nitrogen (N) is essential for the physiological processes of plants. However, the specific mechanisms by which different nitrogen forms influence rice blast pathogenesis remain poorly understood. This study used hydroponic assays to explore how ammonium (NH₄⁺) and nitrate (NO₃⁻) affect rice after inoculation with Magnaporthe oryzae (M. oryzae). The results showed that NH₄⁺, compared to NO₃⁻, significantly reduced disease severity, fungal growth, fungal hyphae number, the expansion capacity of infectious hyphae, and disease-related loss of photosynthesis. Additionally, NH₄⁺ enhanced the expression of defense-related genes, including OsPBZ1, OsCHT1, OsPR1a, and OsPR10. NH₄⁺-treated rice also exhibited higher hydrogen peroxide (H₂O₂) accumulation and increased antioxidant enzyme activities. Moreover, susceptibility to rice blast disease increased when H₂O₂ was scavenged, while a reduction in susceptibility was observed with the application of exogenous H₂O₂. These results suggest that ammonium enhances rice resistance to M. oryzae, potentially through H₂O₂ accumulation. The findings provide valuable insights into how different nitrogen forms affect plant immunity in rice, which is crucial for controlling rice blast and ensuring stable food production.

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铵通过 H2O2 积累增强水稻对 Magnaporthe oryzae 的抗性

氮（N）对植物的生理过程至关重要。然而，人们对不同形式的氮影响稻瘟病发病的具体机制仍然知之甚少。本研究利用水培试验探讨了铵态氮（NH4+）和硝态氮（NO3-）在接种稻瘟病棒状杆菌（M. oryzae）后对水稻的影响。结果表明，与 NO3- 相比，NH4+ 能显著降低病害严重程度、真菌生长、真菌菌丝数量、传染性菌丝的扩展能力以及与病害相关的光合作用损失。此外，NH4+ 还能增强防御相关基因的表达，包括 OsPBZ1、OsCHT1、OsPR1a 和 OsPR10。经 NH4+ 处理的水稻还表现出较高的过氧化氢（H2O2）积累和较高的抗氧化酶活性。此外，清除 H2O2 会增加对稻瘟病的易感性，而施用外源 H2O2 则会降低易感性。这些结果表明，铵可能通过 H2O2 的积累增强了水稻对 M. oryzae 的抗性。这些发现为了解不同氮素形式如何影响水稻的植物免疫力提供了有价值的见解，而植物免疫力对于控制稻瘟病和确保粮食稳定生产至关重要。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.