Deciphering the role of SlWRKY36 and SlWRKY51 in salt stress tolerance via modulating ion homeostasis and proline biosynthesis

IF 5.4 Q1 PLANT SCIENCES
Iqra Mehboob , Sofia Baig , Maria Siddique , Xiaoliang Shan , Ayesha Baig , Mohammad Maroof Shah , Irum Shahzadi , Hongwei Zhao , Shamyla Nawazish , Samina Khalid
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引用次数: 0

Abstract

Soil salinity caused by NaCl is a major challenge to agricultural crops worldwide. For this, two WRKY transcription factors were evaluated for their role in salt stress tolerance in tomato plants (Solanum lycopersicum; Sl). SlWRKY36 and SlWRKY51 provided novel insight into the regulatory mechanism in tomato against salt stress via virus-induced gene silencing (VIGS). Salt stress significantly reduced chlorophyll-a, an abundant form of chlorophyll content to 6.0 and 5.1 mg/g and proline content to 0.06 mg/g and 0.09 mg/g respectively in SlWRKY36 and SlWRKY51 silenced tomato plants. This shows that salt stress affected proline content that act as osmo-protectant and damaged photosynthetic pigments in silenced SlWRKY36 and SlWRKY51 tomato plants. Similarly, the concentrations of Na+/ K+ ratio also showed a significantly higher trend 14 days after salt stress with 5.5 mg/g and 8.9 mg/g concentration at 200 mM for SlWRKY36 and SlWRKY51 showing silencing promotes Na+/K+ ion ratio under salt stress. Also, salt stress responsive genes such as salt overly sensitive SOS1 and Na+/H+ exchanger NHX1 displayed lower transcript level in silenced plants at 200 mM salt stress showing their negative regulation by SlWRKY36 and SlWRKY51 gene silencing. Collectively, these findings suggest for the first time the role of SlWRKY36 and SlWRKY51 as positive regulators of salt stress tolerance by managing ion homeostasis, proline content and photosynthetic machinery via transcriptional reprogramming. Overall, SlWRKY36 and SlWRKY51 were explored as potential candidates for engineering salt tolerance in tomato crop plants.

解密 SlWRKY36 和 SlWRKY51 通过调节离子平衡和脯氨酸生物合成在耐盐胁迫中的作用
由 NaCl 引起的土壤盐碱化是全球农作物面临的一大挑战。为此,研究人员评估了两个 WRKY 转录因子在番茄植物(Solanum lycopersicum; Sl)耐盐胁迫中的作用。SlWRKY36和SlWRKY51通过病毒诱导的基因沉默(VIGS)对番茄抗盐胁迫的调控机制提供了新的见解。在 SlWRKY36 和 SlWRKY51 沉默的番茄植株中,盐胁迫使叶绿素-a(叶绿素的一种丰富形式)含量分别明显降低到 6.0 和 5.1 毫克/克,脯氨酸含量分别降低到 0.06 毫克/克和 0.09 毫克/克。这表明盐胁迫影响了沉默的 SlWRKY36 和 SlWRKY51 番茄植株中作为渗透保护剂的脯氨酸含量,并破坏了光合色素。同样,盐胁迫 14 天后,SlWRKY36 和 SlWRKY51 的 Na+/K+ 比率浓度也呈显著升高趋势,在 200 毫摩尔浓度下分别为 5.5 毫克/克和 8.9 毫克/克,这表明沉默会促进盐胁迫下 Na+/K+ 离子比率的升高。此外,在 200 毫摩尔盐胁迫条件下,沉默植株中的盐胁迫响应基因(如对盐过度敏感的 SOS1 和 Na+/H+ 交换子 NHX1)的转录水平较低,这表明它们受 SlWRKY36 和 SlWRKY51 基因沉默的负调控。总之,这些发现首次表明 SlWRKY36 和 SlWRKY51 通过转录重编程管理离子平衡、脯氨酸含量和光合作用机制,从而对盐胁迫耐受性起到正向调控作用。总之,SlWRKY36 和 SlWRKY51 是番茄作物耐盐工程的潜在候选者。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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