The ultrasonic-assisted enzymatic extraction, characteristics and antioxidant activities of lychee nuclear polysaccharide

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2024-08-21 DOI:10.1016/j.ultsonch.2024.107038

Zongyan Song , Gangliang Huang , Hualiang Huang

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Abstract

A response surface methodology (RSM) and one-factor method were established to investigate the optimum conditions for the extraction of lychee nuclear polysaccharides (LSP) by ultrasonic-assisted pectinase. The content of basic components in polysaccharides was determined. The antioxidant activity was well determined and compared the differences in the activities of the polysaccharides extracted by water extraction (LSP-HW) and those extracted at ultrasound-assisted enzyme (LSP-UAE). The activity of lychee nuclear polysaccharide increased with the increase of concentration. The anti-hydroxyl radical and anti-lipid peroxidation abilities of lychee nuclear polysaccharide were more excellent, and LSP-HW was slightly better than LSP-UAE in terms of activity, but the difference was not significant. In terms of solubility; LSP-HW without deproteinization was the best, followed by LSP-UAE, and the worst was LSP-UAE after deproteinization. The higher the glycoprotein content, the better the solubility of its polysaccharide. Compared with the existing extraction methods, this experiment greatly improved the extraction rate of lychee nuclear polysaccharides and further enriched the antioxidant activities of polysaccharides.

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超声辅助酶法提取荔枝核多糖及其特性和抗氧化活性的研究

建立了响应面法（RSM）和单因素法研究超声波辅助果胶酶提取荔枝核多糖的最佳条件。测定了多糖中基本成分的含量。测定了荔枝核多糖的抗氧化活性，并比较了水提取多糖（LSP-HW）和超声辅助酶提取多糖（LSP-UAE）的抗氧化活性差异。荔枝核多糖的活性随浓度的增加而增加。荔枝核多糖的抗羟基自由基和抗脂质过氧化能力更强，LSP-HW的活性略优于LSP-UAE，但差异不显著。在溶解性方面，未脱蛋白的 LSP-HW 最好，其次是 LSP-UAE，最差的是脱蛋白后的 LSP-UAE。糖蛋白含量越高，其多糖的溶解度越好。与现有的提取方法相比，本实验大大提高了荔枝核多糖的提取率，进一步丰富了多糖的抗氧化活性。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.