Oxidative modulations in platelets stored in SSP+, PAS-G and Tyrode's buffer: a comparative analysis.

Magdaline Christina Rajanand, Anusha Berikai Ananthakrishna, Vani Rajashekaraiah
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Abstract

Background: Platelet additive solutions (PASs) improve the efficacy of stored platelets. Oxidative stress causes storage lesions and platelet functions deteriorate. Studies assessing the influence of oxidative stress on platelets stored in PASs are limited. This study compares variations in platelets in different storage solutions (SSP+, PAS-G and Tyrode's buffer).

Methods: Platelets isolated from the blood of Wistar rats were resuspended in SSP+, PAS-G and Tyrode's buffer and stored for seven days at 22 °C. The markers of platelet metabolism, function, oxidative stress, antioxidant status and viability were analyzed on Days 1, 3, 5 and 7 of storage.

Main results: SSP+ is associated with platelet function, viability and antioxidant defenses (SOD, CAT and GSH); it decreased primary lipid peroxidation products and maintained the susceptible protein groups in reduced state. Platelet function, antioxidant defenses such as SOD and GSH improved, and lipids and thiols were protected from oxidation in PAS-G. SOD and GSH increased, and lipids and thiols were preserved in Tyrode's buffer.

Conclusion: SSP+ and PAS-G are more effective in maintaining platelet efficacy till Day 7 compared to Tyrode's buffer. Thus, PAS-G and SSP+ are better than Tyrode's buffer in terms of platelet responses to oxidative stress during storage. This is the first comparative account on the influence of PASs (SSP+, PAS-G and Tyrode's buffer) on platelets in altering oxidative stress. It provides a comprehensive view of the differential responses of platelets in PASs.

储存在 SSP+、PAS-G 和泰罗德缓冲液中的血小板的氧化调节:比较分析。
背景:血小板添加剂溶液(PAS)可提高储存血小板的功效。氧化应激会导致贮存病变和血小板功能退化。评估氧化应激对储存在血小板添加剂溶液中的血小板的影响的研究十分有限。本研究比较了血小板在不同储存溶液(SSP+、PAS-G 和泰罗德缓冲液)中的变化:方法:将从 Wistar 大鼠血液中分离出的血小板重悬于 SSP+、PAS-G 和泰罗德缓冲液中,并在 22 °C 下储存 7 天。在储存的第 1、3、5 和 7 天分析血小板代谢、功能、氧化应激、抗氧化状态和活力的指标:主要结果:SSP+ 与血小板功能、存活率和抗氧化防御能力(SOD、CAT 和 GSH)有关;它减少了初级脂质过氧化产物,并使易受影响的蛋白质组保持还原状态。在 PAS-G 中,血小板功能、SOD 和 GSH 等抗氧化防御功能得到改善,脂质和硫醇免受氧化。在泰罗德缓冲液中,SOD 和 GSH 增加,脂质和硫醇得到保护:结论:与泰罗德缓冲液相比,SSP+ 和 PAS-G 能更有效地维持血小板功效至第 7 天。因此,就血小板在储存期间对氧化应激的反应而言,PAS-G 和 SSP+ 优于泰罗德缓冲液。这是首次比较研究 PAS(SSP+、PAS-G 和泰罗德缓冲液)在改变氧化应激方面对血小板的影响。它对血小板在 PASs 中的不同反应提供了一个全面的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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