A novel antifungal peptide, SP1.2, from Rhodopseudomonas palustris against the rice blast pathogen

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-08-23 DOI:10.1002/ps.8387

Xiyang Wu, Yingfei Qin, Chenggang Li, Xin Zhang, Xinqiu Tan, Yong Liu, Yue Chen, Deyong Zhang

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引用次数: 0

Abstract

BACKGROUND

Rice blast has a significant detrimental impact on rice yields, so developing efficient biological control technologies is an effective means for rice blast prevention and control. The GroEL protein has proven to be effective at preventing and managing the pathogenicity of rice blast.

RESULTS

Here, we analyzed the amino acid sequence of the GroEL protein and synthesized the ‘60 kDa chaperonin signature’ (350–373 amino acids) peptide SP1.2, which has potent antifungal activity. Notably, the SP1.2 peptide exhibited potent fungicidal activity against Magnaporthe oryzae, effectively inhibiting appressorium germination. Electron microscopy revealed that SP1.2 disrupted the fungal plasma membrane and bound to multiple bioactive phosphoinositides in vitro, triggering the production of reactive oxygen species. Furthermore, it also caused an increase in the acetylation of M. oryzae and induced autophagy in cells. The spray application of SP1.2 significantly reduced the number of disease spots caused by the fungal pathogen M. oryzae in rice, enhancing the defense response of rice plants. Field trials showed that the control effect was 64.59% after spraying SP1.2.

CONCLUSION

Our study illustrates the antifungal activity of the structurally unique SP1.2 peptide against plant fungal pathogens and paves the way for the future development of this class of peptides as antifungal agents. © 2024 Society of Chemical Industry.

Abstract Image

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一种新的抗真菌肽 SP1.2，来自稻瘟病红假单胞菌（Rhodopseudomonas palustris），可对抗稻瘟病病原体。

背景：稻瘟病对水稻产量有重大不利影响，因此开发高效的生物防治技术是防控稻瘟病的有效手段。事实证明，GroEL 蛋白能有效预防和控制稻瘟病的致病性：结果：我们分析了 GroEL 蛋白的氨基酸序列，合成了 "60 kDa 合子蛋白特征"（350-373 氨基酸）多肽 SP1.2，它具有很强的抗真菌活性。值得注意的是，SP1.2 肽对 Magnaporthe oryzae 具有很强的杀真菌活性，能有效抑制附子萌发。电子显微镜显示，SP1.2 在体外破坏了真菌质膜，并与多种生物活性磷脂结合，引发活性氧的产生。此外，SP1.2 还能引起口蘑乙酰化的增加，并诱导细胞自噬。喷施 SP1.2 能显著减少真菌病原体 M. oryzae 在水稻上引起的病斑数量，增强水稻植株的防御反应。田间试验表明，喷施 SP1.2 后的防治效果为 64.59%：我们的研究说明了结构独特的 SP1.2 肽对植物真菌病原体的抗真菌活性，为今后开发这类肽作为抗真菌剂铺平了道路。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.