Biosensing in the optical switch configuration on strong plasmonic gratings enabling differential referenced detection

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2024-08-01 DOI:10.1016/j.sbsr.2024.100681

Emilie Laffont , Arnaud Valour , Nicolas Crespo-Monteiro , Pierre Berini , Yves Jourlin

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引用次数: 0

Abstract

A deep gold-coated sinusoidal grating is proposed as a transducer for label-free real-time biosensing, operating in a new configuration based on the optical switch effect, which produces complementary optical outputs enabling differential and referenced detection. Biosensing experiments are reported for the first time on this platform, using immunoassays involving biospecific pairs consisting of bovine serum albumin and its antibody, and human serum albumin and its antibody. Direct and sandwich immunoassays are demonstrated along with negative controls. A limit of detection of 6 pg/mm² was obtained. A theoretical model correlating the variation in the differential referenced output optical signal with adlayer growth is presented and supports the experimental results. The proposed detection device operating in the optical switch configuration makes a promising case for point-of-care detection applications because the differential detection of two diffracted orders enables common noise suppression and robust interrogation.

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在强等离子光栅的光学开关配置中进行生物传感，实现差分参考检测

我们提出了一种深金涂层正弦光栅，作为无标记实时生物传感的换能器，它以基于光学开关效应的新配置运行，产生互补光学输出，从而实现差分和参考检测。首次报道了在该平台上进行的生物传感实验，使用的免疫测定涉及由牛血清白蛋白及其抗体和人血清白蛋白及其抗体组成的生物特异性配对。直接免疫测定和夹心免疫测定以及阴性对照都得到了验证。检测限为 6 pg/mm2。实验提出了一个理论模型，该模型将差分参考输出光信号的变化与吸附层的生长联系起来，并支持实验结果。由于两个衍射阶次的差分检测可实现常见的噪声抑制和稳健的询问，因此，以光学开关配置运行的拟议检测设备在护理点检测应用中大有可为。

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.