Conquering Limitations: Exploring the Factors that Drive Successful Agrobacterium-Mediated Genetic Transformation of Recalcitrant Plant Species.

IF 2.5 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-08-01 Epub Date: 2024-08-23 DOI:10.1007/s12033-024-01247-x

Nafiseh Aboofazeli, Solmaz Khosravi, Hedayat Bagheri, Stephen F Chandler, Shen Q Pan, Pejman Azadi

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Abstract

Agrobacterium-mediated transformation is a preferred method for genetic engineering and genome editing of plants due to its numerous advantages, although not all species exhibit transformability. Genetic engineering and plant genome editing methods are technically challenging in recalcitrant crop plants. Factors affecting the poor rate of transformation in such species include host genotype, Agrobacterium genotype, type of explant, physiological condition of the explant, vector, selectable marker, inoculation method, chemical additives, antioxidative compounds, transformation-enhancing compounds, medium formulation, optimization of culture conditions, and pre-treatments. This review provides novel insights into the key factors involved in gene transfer facilitated by Agrobacterium and proposes potential solutions to overcome existing barriers to transformation in recalcitrant species, thereby contributing to improvement programs for these species. This review introduces the key factors that impact the effectiveness of a molecular breeding program using Agrobacterium-mediated transformation, specifically focusing on recalcitrant plant species.

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征服限制：探索促使农杆菌介导的难治性植物物种基因转化成功的因素。

农杆菌介导的转化因其众多优点而成为植物基因工程和基因组编辑的首选方法，但并非所有物种都具有可转化性。基因工程和植物基因组编辑方法对于顽劣的作物植物来说具有技术挑战性。影响此类物种转化率低的因素包括宿主基因型、农杆菌基因型、外植体类型、外植体生理条件、载体、可选择标记、接种方法、化学添加剂、抗氧化化合物、转化增强化合物、培养基配方、培养条件优化和预处理。本综述对农杆菌促进基因转移所涉及的关键因素提供了新的见解，并提出了潜在的解决方案，以克服现有的难以转化物种的转化障碍，从而促进这些物种的改良计划。本综述介绍了影响利用农杆菌介导转化的分子育种计划有效性的关键因素，特别侧重于难以转化的植物物种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.