Eco-toxicity assessment of polypropylene microplastics in juvenile zebrafish (Danio rerio)

IF 3.5 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES
Suresh Priyadharshini , Jeyaraj Jeyavani , Khalid A. Al-Ghanim , Marimuthu Govindarajan , Sivashanmugam Karthikeyan , Baskaralingam Vaseeharan
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Abstract

In recent years, everyone has recognized microplastics as an emerging contaminant in aquatic ecosystems. Polypropylene is one of the dominant pollutants. The purpose of this study was to examine the effects of exposing zebrafish (Danio rerio) to water with various concentrations of polypropylene microplastics (11.86 ± 44.62 μm), including control (0 mg/L), group 1 (1 mg/L), group 2 (10 mg/L), and group 3 (100 mg/L) for up to 28 days (chronic exposure). The bioaccumulation of microplastics in the tract was noted after 28 days. From the experimental groups, blood and detoxifying organs of the liver and brain were collected. Using liver tissues evaluated the toxic effects by crucial biomarkers such as reactive oxygen species, anti-oxidant parameters, oxidative effects in protein & lipids, total protein content and free amino acid level. The study revealed that the bioaccumulation of microplastics in the organisms is a reflection of the oxidative stress and liver tissue damage experienced by the group exposed to microplastics. Also, apoptosis of blood cells was observed in the treated group as well as increased the neurotransmitter enzyme acetylcholine esterase activity based on exposure concentration-dependent manner. The overall results indicated bioaccumulation of microplastics in the gut, which led to increased ROS levels. This consequently affected antioxidant biomarkers, ultimately causing oxidation of biomolecules and liver tissue injury, as evidenced by histological analysis. This study concludes that chronic ingestion of microplastics causes considerable effects on population fitness in the aquatic environment, as well as other ecological complications, and is also critical to understand the magnitude of these contaminants' influence on ichthyofauna.

Abstract Image

聚丙烯微塑料对幼年斑马鱼(Danio rerio)的生态毒性评估。
近年来,大家都认识到微塑料是水生生态系统中新出现的污染物。聚丙烯是主要污染物之一。本研究的目的是考察将斑马鱼(Danio rerio)暴露在含有不同浓度聚丙烯微塑料(11.86 ± 44.62 μm)的水中长达 28 天(慢性暴露)的影响,包括对照组(0 mg/L)、第 1 组(1 mg/L)、第 2 组(10 mg/L)和第 3 组(100 mg/L)。28 天后,观察微塑料在肠道中的生物累积情况。收集了实验组的血液以及肝脏和大脑的解毒器官。利用肝脏组织,通过活性氧、抗氧化参数、蛋白质和脂质中的氧化作用、总蛋白质含量和游离氨基酸水平等关键生物标志物评估毒性效应。研究显示,微塑料在生物体内的生物累积反映了暴露于微塑料的群体所经历的氧化应激和肝组织损伤。此外,根据暴露浓度依赖性方式,在处理组中观察到血细胞凋亡以及神经递质酶乙酰胆碱酯酶活性增加。总体结果表明,微塑料在肠道中的生物累积导致了 ROS 水平的升高。组织学分析表明,这影响了抗氧化生物标志物,最终导致生物大分子氧化和肝组织损伤。这项研究的结论是,长期摄入微塑料会对水生环境中的种群适应性造成相当大的影响,并引发其他生态并发症,这对于了解这些污染物对鱼类的影响程度也至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of contaminant hydrology
Journal of contaminant hydrology 环境科学-地球科学综合
CiteScore
6.80
自引率
2.80%
发文量
129
审稿时长
68 days
期刊介绍: The Journal of Contaminant Hydrology is an international journal publishing scientific articles pertaining to the contamination of subsurface water resources. Emphasis is placed on investigations of the physical, chemical, and biological processes influencing the behavior and fate of organic and inorganic contaminants in the unsaturated (vadose) and saturated (groundwater) zones, as well as at groundwater-surface water interfaces. The ecological impacts of contaminants transported both from and to aquifers are of interest. Articles on contamination of surface water only, without a link to groundwater, are out of the scope. Broad latitude is allowed in identifying contaminants of interest, and include legacy and emerging pollutants, nutrients, nanoparticles, pathogenic microorganisms (e.g., bacteria, viruses, protozoa), microplastics, and various constituents associated with energy production (e.g., methane, carbon dioxide, hydrogen sulfide). The journal''s scope embraces a wide range of topics including: experimental investigations of contaminant sorption, diffusion, transformation, volatilization and transport in the surface and subsurface; characterization of soil and aquifer properties only as they influence contaminant behavior; development and testing of mathematical models of contaminant behaviour; innovative techniques for restoration of contaminated sites; development of new tools or techniques for monitoring the extent of soil and groundwater contamination; transformation of contaminants in the hyporheic zone; effects of contaminants traversing the hyporheic zone on surface water and groundwater ecosystems; subsurface carbon sequestration and/or turnover; and migration of fluids associated with energy production into groundwater.
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