Assessment of Tumor Cell Invasion and Radiotherapy Response in Experimental Glioma by Magnetic Resonance Elastography

IF 3.3 2区医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Journal of Magnetic Resonance Imaging Pub Date : 2024-08-23 DOI:10.1002/jmri.29567

Hannah Fels-Palesandro MSc, Sophie Heuer MD, Berin Boztepe MSc, Yannik Streibel, Johannes Ungermann BSc, Chenchen Pan PhD, Jonas G. Scheck, Manuel Fischer, Volker J. Sturm PhD, Daniel D. Azorín PhD, Kianush Karimian-Jazi MD, Giacomo Annio PhD, Amir Abdollahi MD, PhD, Ina Weidenfeld PhD, Wolfgang Wick MD, Varun Venkataramani MD, PhD, Sabine Heiland PhD, Frank Winkler MD, Martin Bendszus MD, Ralph Sinkus PhD, Michael O. Breckwoldt MD, PhD, Katharina Schregel MD

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引用次数: 0

Abstract

Background

Gliomas are highly invasive brain neoplasms. MRI is the most important tool to diagnose and monitor glioma but has shortcomings. In particular, the assessment of tumor cell invasion is insufficient. This is a clinical dilemma, as recurrence can arise from MRI-occult glioma cell invasion.

Hypothesis

Tumor cell invasion, tumor growth and radiotherapy alter the brain parenchymal microstructure and thus are assessable by diffusion tensor imaging (DTI) and MR elastography (MRE).

Study Type

Experimental, animal model.

Animal Model

Twenty-three male NMRI nude mice orthotopically implanted with S24 patient-derived glioma cells (experimental mice) and 9 NMRI nude mice stereotactically injected with 1 μL PBS (sham-injected mice).

Field Strength/Sequence

2D and 3D T2-weighted rapid acquisition with refocused echoes (RARE), 2D echo planar imaging (EPI) DTI, 2D multi-slice multi-echo (MSME) T2 relaxometry, 3D MSME MRE at 900 Hz acquired at 9.4 T (675 mT/m gradient strength).

Assessment

Longitudinal 4-weekly imaging was performed for up to 4 months. Tumor volume was assessed in experimental mice (n = 10 treatment-control, n = 13 radiotherapy). The radiotherapy subgroup and 5 sham-injected mice underwent irradiation (3 × 6 Gy) 9 weeks post-implantation/sham injection. MRI-/MRE-parameters were assessed in the corpus callosum and tumor core/injection tract. Imaging data were correlated to light sheet microscopy (LSM) and histology.

Statistical Tests

Paired and unpaired t-tests, a P-value ≤0.05 was considered significant.

Results

From week 4 to 8, a significant callosal stiffening (4.44 ± 0.22 vs. 5.31 ± 0.29 kPa) was detected correlating with LSM-proven tumor cell invasion. This was occult to all other imaging metrics. Histologically proven tissue destruction in the tumor core led to an increased T2 relaxation time (41.65 ± 0.34 vs. 44.83 ± 0.66 msec) and ADC (610.2 ± 12.27 vs. 711.2 ± 13.42 × 10⁻⁶ mm²/s) and a softening (5.51 ± 0.30 vs. 4.24 ± 0.29 kPa) from week 8 to 12. Radiotherapy slowed tumor progression.

Data Conclusion

MRE is promising for the assessment of key glioma characteristics.

Evidence Level

Technical Efficacy

Stage 2

Abstract Image

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通过磁共振弹性成像评估实验性胶质瘤的肿瘤细胞侵袭和放疗反应

背景：胶质瘤是侵袭性很强的脑肿瘤。磁共振成像是诊断和监测胶质瘤的最重要工具，但也有不足之处。尤其是对肿瘤细胞侵袭的评估不足。这是一个临床难题，因为核磁共振成像发现的胶质瘤细胞侵犯可能导致复发：假设：肿瘤细胞入侵、肿瘤生长和放疗会改变脑实质的微观结构，因此可通过弥散张量成像（DTI）和磁共振弹性成像（MRE）进行评估：实验，动物模型：23 只雄性 NMRI 裸鼠正位植入 S24 患者衍生的胶质瘤细胞（实验小鼠），9 只 NMRI 裸鼠立体注射 1 μL PBS（假注射小鼠）。场强/序列：二维和三维 T2 加权快速采集再聚焦回波（RARE）、二维回波平面成像（EPI）DTI、二维多切片多回波（MSME）T2 弛豫测量、在 9.4 T（675 mT/m 梯度强度）下以 900 Hz 采集的三维 MSME MRE：每 4 周进行一次纵向成像，最长持续 4 个月。对实验小鼠的肿瘤体积进行评估（治疗-对照组 10 只，放疗组 13 只）。放疗亚组和 5 只假注射小鼠在植入/注射后 9 周接受了照射（3 × 6 Gy）。对胼胝体和肿瘤核心/注射道的核磁共振成像/MRE参数进行了评估。成像数据与光片显微镜（LSM）和组织学相关联：统计检验：配对和非配对t检验，P值≤0.05为显著：结果：从第4周到第8周，发现胼胝体明显变硬（4.44 ± 0.22 vs. 5.31 ± 0.29 kPa），这与LSM证实的肿瘤细胞侵犯相关。这与所有其他成像指标无关。组织学证实的肿瘤核心组织破坏导致第8周至第12周的T2弛豫时间（41.65 ± 0.34 vs. 44.83 ± 0.66毫秒）和ADC（610.2 ± 12.27 vs. 711.2 ± 13.42 × 10-6 mm2/s）增加以及软化（5.51 ± 0.30 vs. 4.24 ± 0.29 kPa）。放疗减缓了肿瘤的进展：数据结论：MRE 有助于评估胶质瘤的主要特征：不适用技术功效：第 2 阶段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Magnetic Resonance Imaging 医学-核医学

CiteScore

9.70

自引率

6.80%

发文量

494

审稿时长

2 months

期刊介绍： The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.