Development and validation of a novel enzyme-linked immunosorbent assay for the differentiation of tick-borne encephalitis infections caused by different virus subtypes.

IF 5.4 2区医学 Q1 INFECTIOUS DISEASES

Infection Pub Date : 2024-08-23 DOI:10.1007/s15010-024-02370-2

Zane Freimane, Gerhard Dobler, Lidia Chitimia-Dobler, Guntis Karelis, Philipp Girl, Sanita Kuzmane, Oksana Savicka, Wilhelm Erber, Dace Zavadska

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Abstract

Objectives: Tick-borne encephalitis (TBE) is an infection caused by the tick-borne encephalitis virus (TBEV) that can lead to symptoms of central nervous system inflammation. There are five subtypes of TBEV, three of which - European, Siberian and Far Eastern - occur in Europe. As it is thought that different subtype infections exhibit varying clinical courses and outcomes, serological differentiation of the virus subtypes is clearly important. However, to date, this has proved difficult to achieve.

Methods: An ELISA format was developed based on TBE virus NS1 antigen against the European, Siberian and Far Eastern subtype. The three NS1 antigens were biotechnologically produced in a human cell line and used for ELISA coating. Sera from German (European subtype) and Russian (Siberian and/or Far Eastern subtypes) TBE patients with positive TBEV IgG were used to test the reactivity against these three NS1 antigens.

Results: Testing of 23 German and 32 Russian TBEV IgG-positive sera showed that the ELISA was able to differentiate between TBEV European subtype and TBEV Siberian and Far Eastern subtype infections.

Conclusions: In geographical areas where two or more TBEV subtype infections can occur, the NS1-IgG ELISA developed here constitutes an important diagnostic tool to differentiate between European subtype infections and Siberian/Far Eastern subtype infections and to use the new assay for epidemiological studies to clarify the importance of particular subtype infections in an area. Consequently, it may help to better describe and anticipate the clinical courses and outcomes of particular TBEV subtype infections.

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开发并验证一种新型酶联免疫吸附试验，用于区分由不同病毒亚型引起的蜱传脑炎感染。

目的：蜱传脑炎（TBE）是由蜱传脑炎病毒（TBEV）引起的感染，可导致中枢神经系统炎症症状。TBEV 有五种亚型，其中三种--欧洲型、西伯利亚型和远东型--出现在欧洲。据认为，不同亚型的感染表现出不同的临床过程和结果，因此通过血清学区分病毒亚型显然非常重要。然而，迄今为止，这一点还很难实现：方法：根据针对欧洲、西伯利亚和远东亚型的 TBE 病毒 NS1 抗原开发了一种 ELISA 方法。这三种 NS1 抗原是通过生物技术在人类细胞系中产生的，并用于 ELISA 涂布。用德国（欧洲亚型）和俄罗斯（西伯利亚和/或远东亚型）TBEV IgG 阳性的 TBEV 患者的血清来检测对这三种 NS1 抗原的反应性：对 23 份德国 TBEV IgG 阳性血清和 32 份俄罗斯 TBEV IgG 阳性血清的检测结果表明，ELISA 能够区分欧洲亚型 TBEV 感染和西伯利亚及远东亚型 TBEV 感染：结论：在可能出现两种或两种以上 TBEV 亚型感染的地区，本文开发的 NS1-IgG ELISA 是一种重要的诊断工具，可用于区分欧洲亚型感染和西伯利亚/远东亚型感染，还可用于流行病学研究，以明确特定亚型感染在某一地区的重要性。因此，它有助于更好地描述和预测特定 TBEV 亚型感染的临床过程和结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection 医学-传染病学

CiteScore

12.50

自引率

1.30%

发文量

224

审稿时长

6-12 weeks

期刊介绍： Infection is a journal dedicated to serving as a global forum for the presentation and discussion of clinically relevant information on infectious diseases. Its primary goal is to engage readers and contributors from various regions around the world in the exchange of knowledge about the etiology, pathogenesis, diagnosis, and treatment of infectious diseases, both in outpatient and inpatient settings. The journal covers a wide range of topics, including: Etiology: The study of the causes of infectious diseases. Pathogenesis: The process by which an infectious agent causes disease. Diagnosis: The methods and techniques used to identify infectious diseases. Treatment: The medical interventions and strategies employed to treat infectious diseases. Public Health: Issues of local, regional, or international significance related to infectious diseases, including prevention, control, and management strategies. Hospital Epidemiology: The study of the spread of infectious diseases within healthcare settings and the measures to prevent nosocomial infections. In addition to these, Infection also includes a specialized "Images" section, which focuses on high-quality visual content, such as images, photographs, and microscopic slides, accompanied by brief abstracts. This section is designed to highlight the clinical and diagnostic value of visual aids in the field of infectious diseases, as many conditions present with characteristic clinical signs that can be diagnosed through inspection, and imaging and microscopy are crucial for accurate diagnosis. The journal's comprehensive approach ensures that it remains a valuable resource for healthcare professionals and researchers in the field of infectious diseases.