Maternal Spargel/dPGC-1 is critical for embryonic development and influences chorion gene amplification via Cyclin E activity

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Md Shah Jalal, Atanu Duttaroy
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引用次数: 0

Abstract

The function of spargel/dPGC-1 in Drosophila oogenesis has been unequivocally established. Here, we sought to assess whether Spargel protein or RNA is essential for developmentally competent eggs. The trans-heterozygotic combination of two spargel mutant alleles allowed us to decrease Spargel expression to very low levels. Using this model, we now demonstrated the requirement for Spargel in eggshell patterning and embryonic development, which led us to establish that spargel is a maternal effect gene. Further examination of Spargel's potential mechanism of action in eggshell biogenesis revealed that low levels of Spargel in the adult ovary cause diminished Cyclin E activity, resulting in reduced chorion gene amplification levels, leading to eggshell biogenesis defects. Thus, another novel role for spargel/dPGC-1 is exposed whereby, through Cyclin E activity, this conserved transcriptional coactivator regulates the chorion gene amplification process.

Abstract Image

母体 Spargel/dPGC-1 对胚胎发育至关重要,并通过 Cyclin E 的活性影响绒毛膜基因扩增。
spargel/dPGC-1 在果蝇卵子发生过程中的功能已被明确确定。在这里,我们试图评估 Spargel 蛋白或 RNA 是否是发育合格的卵子所必需的。通过两个 spargel 突变等位基因的反式杂合组合,我们可以将 Spargel 的表达降低到非常低的水平。利用这一模型,我们证明了 Spargel 在蛋壳形态和胚胎发育中的必要性,从而确定了 Spargel 是一种母性效应基因。进一步研究 Spargel 在蛋壳生物形成过程中的潜在作用机制发现,成体卵巢中低水平的 Spargel 会导致 Cyclin E 活性降低,从而导致绒毛膜基因扩增水平降低,导致蛋壳生物形成缺陷。由此可见,Spargel/dPGC-1 的另一个新作用是,通过 Cyclin E 的活性,这种保守的转录辅激活因子可调节绒毛膜基因扩增过程。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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