Integrative workflows for the characterization of hydrophobin and cerato-platanin in the marine fungus Paradendryphiella salina

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-08-23 DOI:10.1007/s00203-024-04087-0

Catalina Landeta, David Medina-Ortiz, Natalia Escobar, Iván Valdez, María Paz González-Troncoso, Diego Álvares-Saravia, Jacqueline Aldridge, Carlos Gómez, María Elena Lienqueo

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Abstract

Hydrophobins (HFBs) and cerato-platanins (CPs) are surface-active extracellular proteins produced by filamentous fungi. This study identified two HFB genes (pshyd1 and pshyd2) and one CP gene (pscp) in the marine fungus Paradendryphiella salina. The proteins PsCP, PsHYD2, and PsHYD1 had molecular weights of 12.70, 6.62, and 5.98 kDa, respectively, with isoelectric points below 7. PsHYD1 and PsHYD2 showed hydrophobicity (GRAVY score 0.462), while PsCP was hydrophilic (GRAVY score − 0.202). Stability indices indicated in-solution stability. Mass spectrometry identified 2,922 proteins, including CP but not HFB proteins. qPCR revealed differential gene expression influenced by developmental stage and substrate, with pshyd1 consistently expressed. These findings suggest P. salina’s adaptation to marine ecosystems with fewer hydrophobin genes than other fungi but capable of producing surface-active proteins from seaweed carbohydrates. These proteins have potential applications in medical biocoatings, food industry foam stabilizers, and environmental bioremediation.

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海洋真菌 Paradendryphiella salina 中疏水素和陶瓷肽表征的整合工作流程。

疏水性蛋白（HFBs）和角蛋白（CPs）是丝状真菌产生的具有表面活性的胞外蛋白质。本研究发现了海洋真菌 Paradendryphiella salina 中的两个 HFB 基因（pshyd1 和 pshyd2）和一个 CP 基因（pscp）。蛋白质 PsCP、PsHYD2 和 PsHYD1 的分子量分别为 12.70、6.62 和 5.98 kDa，等电点低于 7。PsHYD1 和 PsHYD2 具有疏水性（GRAVY 得分为 0.462），而 PsCP 具有亲水性（GRAVY 得分为 -0.202）。稳定性指数显示了溶液稳定性。质谱分析鉴定出 2,922 种蛋白质，其中包括 CP 蛋白，但不包括 HFB 蛋白。qPCR 揭示了受发育阶段和底物影响的不同基因表达，其中 pshyd1 始终表达。这些发现表明，与其他真菌相比，盐池蛙适应海洋生态系统的亲水蛋白基因较少，但能从海藻碳水化合物中产生表面活性蛋白。这些蛋白质有望应用于医疗生物涂料、食品工业泡沫稳定剂和环境生物修复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.