Hyperautofluorescent material inside areas of macular atrophy may reveal non-lipofuscin fluorophores in late stage AMD.

IF 3 3区医学 Q1 OPHTHALMOLOGY

Acta Ophthalmologica Pub Date : 2024-08-23 DOI:10.1111/aos.16752

Melih Tarhan, Daniel Meller, Martin Hammer

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引用次数: 0

Abstract

Purpose: To characterize fundus autofluorescence (FAF) in complete (cRORA) and incomplete retinal pigment epithelium and outer retinal atrophy (iRORA) by fluorescence lifetime imaging ophthalmology (FLIO).

Methods: Overall, 98 macular atrophy (MA) lesions in 42 eyes of 37 age-related macular degeneration (AMD) patients (mean age: 80.9 ± 5.8 years), 25 of them classified as iRORA and 73 as cRORA by OCT, were investigated by FLIO in a short (SSC: 498-560 nm) and a long wavelength channel (LSC: 560-720 nm). Differences of FAF lifetimes and peak emission wavelength (PEW) between atrophic lesions and intact retinal pigment epithelium (RPE) in the outer ring of the ETDRS grid were considered.

Results: FAF lifetimes in MA were longer and PEW were significantly (p < 0.001) shorter than in intact RPE by 112 ± 78 ps (SSC), 91 ± 64 ps (LSC), 27 ± 18 nm (PEW) in iRORA and by 227 ± 112 ps (SSC), 167 ± 81 ps (LSC), and 54 ± 17 nm (PEW) in cRORA. 37% of iRORA and 24% of cRORA were hyperautofluorescent in SSC. Persistent sub-RPE-BL material in MA was newly found as a hyperautofluorescent entity with lifetimes considerably longer than that of drusen and RPE.

Conclusions: Despite RPE and, thus, lipofuscin are greatly absent in MA, considerable FAF, preferably at short wavelengths, was found in those lesions. Drusen, persistent sub-RPE-BL material, basal laminar deposits, persistent activated RPE, and sclera were identified as putative sources of this fluorescence. FLIO can help to characterize respective fluorophores.

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黄斑萎缩区域内的高荧光物质可能会显示 AMD 晚期的非脂褐素荧光团。

目的：通过荧光寿命成像眼科学（FLIO）描述完全性（cRORA）和不完全性视网膜色素上皮和外层视网膜萎缩（iRORA）的眼底自发荧光（FAF）特征：在短波长通道（SSC：498-560 nm）和长波长通道（LSC：560-720 nm）中使用荧光寿命成像（FLIO）对37名年龄相关性黄斑变性（AMD）患者（平均年龄：80.9 ± 5.8岁）的42只眼睛中的98个黄斑萎缩（MA）病灶进行了研究，其中25个病灶被OCT归类为iRORA，73个病灶被OCT归类为cRORA。研究考虑了 ETDRS 网格外圈中萎缩病变和完整视网膜色素上皮（RPE）之间 FAF 寿命和峰值发射波长（PEW）的差异：结果：MA 的 FAF 寿命更长，PEW 明显（p 结论：MA 的 FAF 寿命更长，PEW 明显（p）：尽管在 MA 中 RPE 和脂褐素大量缺失，但在这些病变中发现了相当多的 FAF，尤其是在短波长下。眼底出血、持续的 RPE-BL 下物质、基底层沉积、持续活化的 RPE 和巩膜被确定为这种荧光的可能来源。FLIO 可帮助确定各荧光团的特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.