Antifeedant and growth inhibitory effects of extracts from Ajania potaninii and A. fruticulosa against Tribolium castaneum (Coleoptera: Tenebrionidae)

Abstract

This study aimed to evaluate the nutritional and antifeedant indices of Tribolium castaneum in response to the compounds and main chemical constituents of Ajania potaninii and A. fruticulosa. These mainly includes essential oils and their major ingredients (eucalyptol, camphor, myrtenol, verbenol, and borneol), as well as nonvolatile compounds. The A. fruticulosa and A. potaninii EOs exhibited clear antifeedant activity against T. castaneum adults at a 2 mg/L concentration, with FDI of 50% and 60%, respectively. Among the nonvolatile compounds, sesamin and eriodictyol exhibit strong antifeedant activities against T. castaneum adults at the same concentration, with an FDI of 90.57% and 88.68% after 72 h, respectively. For larvae, A. fruticulosa and A. potaninii EOs also resulted in antifeedant activity (respective FDI: 57.08% and 55.63%). In addition, sesamin and eriodictyol had exceptionally potent antifeedant activity against T. castaneum larvae, with FDI values 90.71% and 96.17%, respectively. In nutritional studies, the reductions in relative food consumption rate (RCR) of T. castaneum were more pronounced for 1 mg/L and 2 mg/L of either eucalyptol or sesamin. However, at greater concentrations, the conversion efficiency of eucalyptol and sesamin to ingested food in T. castaneum was significantly improved. Eucalyptol and sesamin showed a dose-dependent FDI for this insect. Considering the significant anti-insect activities, the EOs, main compounds, and non-volatile compounds of both A. potaninii and A. fruticulosa might be useful in devising integrated pest strategies, providing a promising perspective for the comprehensive use of natural plant resources from the Ajania genus.