The OsGAPC1-OsSGL module negatively regulates salt tolerance by mediating abscisic acid biosynthesis in rice

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-08-21 DOI:10.1111/nph.20061

Lingli Jiang, Weiyu Xiao, Huiping Chen, Yinyao Qi, Xinyu Kuang, Jiahui Shi, Zhenming Liu, Jianzhong Cao, Qinlu Lin, Feng Yu, Long Wang

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引用次数: 0

Abstract

Plants frequently encounter adverse conditions and stress during their lives. Abscisic acid (ABA) plays a crucial role in response to salt stress, and dynamic regulation of ABA levels is essential for plant growth and stress resistance.
In this study, we identified a transcription factor, OsSGL (Oryza sativa Stress tolerance and Grain Length), which acts as a negative regulator in salt stress, controlling ABA synthesis. OsSGL-overexpressing and mutant materials exhibited sensitivity and tolerance to salt stress, respectively. Notably, under salt treatment, several ABA-related genes, including the ABA synthesis enzyme OsNCED3 and the ABA response gene OsRAB21, were bound by OsSGL, leading to the inhibition of their transcription.
Additionally, we found that a key enzyme involved in glycolysis, OsGAPC1, interacted with OsSGL and enhanced the inhibitory effect of OsSGL on OsNCED3. Upon salt stress, OsGAPC1 underwent acetylation and then translocated from the nucleus to the cytoplasm, partially alleviating the inhibitory effect of OsSGL on OsNCED3. Identification of the OsGAPC1-OsSGL module revealed a negative regulatory mechanism involved in the response of rice to salt stress.
This discovery provides insight into the dynamic regulation of ABA synthesis in plants under salt stress conditions, highlighting the delicate balance between stress resistance and growth regulation.

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OsGAPC1-OsSGL 模块通过介导水稻中脱落酸的生物合成来负向调节耐盐性。

植物在一生中经常会遇到不利条件和胁迫。脱落酸（ABA）在盐胁迫响应中起着至关重要的作用，而 ABA 水平的动态调节对植物的生长和抗逆性至关重要。在这项研究中，我们发现了一个转录因子 OsSGL（Oryza sativa Stress tolerance and Grain Length），它在盐胁迫中起负调控作用，控制 ABA 的合成。OsSGL过表达材料和突变体材料分别表现出对盐胁迫的敏感性和耐受性。值得注意的是，在盐胁迫下，多个 ABA 相关基因（包括 ABA 合成酶 OsNCED3 和 ABA 响应基因 OsRAB21）被 OsSGL 结合，导致其转录受到抑制。此外，我们还发现参与糖酵解的关键酶 OsGAPC1 与 OsSGL 相互作用，增强了 OsSGL 对 OsNCED3 的抑制作用。盐胁迫时，OsGAPC1发生乙酰化，然后从细胞核转位到细胞质，部分缓解了OsSGL对OsNCED3的抑制作用。OsGAPC1-OsSGL 模块的鉴定揭示了水稻对盐胁迫响应的负调控机制。这一发现深入揭示了植物在盐胁迫条件下 ABA 合成的动态调控，凸显了抗逆性和生长调控之间的微妙平衡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.