Mesenchymal Stem Cells Increase Resistance Against Ventricular Arrhythmias Provoked in Rats with Myocardial Infarction.

IF 4.5 3区医学 Q2 CELL & TISSUE ENGINEERING

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-08-22 DOI:10.1007/s12015-024-10773-9

Larissa Emília Seibt, Ednei Luiz Antonio, Ighor Luiz AzevedoTeixeira, Helenita Antonia de Oliveira, André Rodrigues Lourenço Dias, Luis Felipe Neves Dos Santos, Andrey Jorge Serra

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Abstract

This study evaluated the role of the mesenchymal stem cells derived from adipose tissue (MSCs) in provoked ventricular arrhythmias (VAs) in animals with myocardial infarction (MI). The experimental groups were: sham, subjected to sham surgery and intramyocardial saline injection; MIV, infarcted rats subjected to intramyocardial saline injection; MI + MSCs, infarcted rats subjected to intramyocardial MSCs injection. Injections were performed two days after infarction and the arrhythmogenic inducibility experiment was performed the next day. Only 35% of the MI + MSCs group developed VAs, while the one in the MIV group was 65%. The proportion of nonsustained ventricular tachycardia, sustained tachycardia, and ventricular fibrillation was similar between the infarcted groups, but MSCs animals had shorter duration of nonsustained ventricular tachycardia. However, MSCs increased connexin 43 content in the remote area, even above the levels found in the sham group. MSCs prevented the increase of IL-1β in the different areas of the myocardium. There was higher carbonylation and content of 4-hydroxynonenal (4-HNE, a marker of lipoperoxidation) in the myocardium of infarcted rats, but MSCs attenuated the increase of 4-HNE in the infarcted area. In conclusion, MSCs have a protective effect against the development of arrhythmias, but do not imply a significant benefit for animals that have developed VAs. It is possible to think that the cardioprotection of MSCs involves anti-inflammatory/oxidative actions and improvement in the formation of communicating junctions.Graphical abstract.

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间充质干细胞增强心肌梗死大鼠对室性心律失常的抵抗力

本研究评估了脂肪组织间充质干细胞（MSCs）在心肌梗死（MI）动物诱发室性心律失常（VAs）中的作用。实验组为：假组，接受假手术和心肌内生理盐水注射；MIV组，心肌梗死大鼠接受心肌内生理盐水注射；MI + MSCs组，心肌梗死大鼠接受心肌内间叶干细胞注射。心肌梗死两天后进行注射，第二天进行致心律失常诱导实验。心肌梗死+间充质干细胞组仅有35%的大鼠出现室性早搏，而MIV组则为65%。梗死组非持续性室速、持续性室速和室颤的比例相似，但间叶干细胞动物非持续性室速的持续时间较短。然而，间叶干细胞增加了远端区域的连索 43 含量，甚至超过了假体组的水平。间充质干细胞阻止了IL-1β在心肌不同区域的增加。梗死大鼠心肌中4-羟基壬烯醛（4-HNE，一种脂质过氧化标记物）的羰基化和含量较高，但间叶干细胞可减轻梗死区域4-HNE的增加。总之，间充质干细胞对心律失常的发生有保护作用，但并不意味着对发生VA的动物有明显的益处。可以认为间叶干细胞对心脏的保护作用包括抗炎/抗氧化作用和改善沟通连接的形成。

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来源期刊

Stem Cell Reviews and Reports 医学-细胞生物学

CiteScore

9.30

自引率

4.20%

发文量

审稿时长

3 months

期刊介绍： The purpose of Stem Cell Reviews and Reports is to cover contemporary and emerging areas in stem cell research and regenerative medicine. The journal will consider for publication: i) solicited or unsolicited reviews of topical areas of stem cell biology that highlight, critique and synthesize recent important findings in the field. ii) full length and short reports presenting original experimental work. iii) translational stem cell studies describing results of clinical trials using stem cells as therapeutics. iv) papers focused on diseases of stem cells. v) hypothesis and commentary articles as opinion-based pieces in which authors can propose a new theory, interpretation of a controversial area in stem cell biology, or a stem cell biology question or paradigm. These articles contain more speculation than reviews, but they should be based on solid rationale. vi) protocols as peer-reviewed procedures that provide step-by-step descriptions, outlined in sufficient detail, so that both experts and novices can apply them to their own research. vii) letters to the editor and correspondence. In order to facilitate this exchange of scientific information and exciting novel ideas, the journal has created five thematic sections, focusing on: i) the role of adult stem cells in tissue regeneration; ii) progress in research on induced pluripotent stem cells, embryonic stem cells and mechanism governing embryogenesis and tissue development; iii) the role of microenvironment and extracellular microvesicles in directing the fate of stem cells; iv) mechanisms of stem cell trafficking, stem cell mobilization and homing with special emphasis on hematopoiesis; v) the role of stem cells in aging processes and cancerogenesis.