Transcriptome analysis of Sesuvium portulacastrum L. uncovers key genes and pathways involved in root formation in response to low-temperature stress.

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jingyi Yang, Shiyu Lin, Yinghan Shen, Jingtao Ye, Xiamin Jiang, Sheng Li, Maowang Jiang
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Abstract

Sesuvium portulacastrum L., a perennial facultative halophyte, is extensively distributed across tropical and subtropical coastal regions. Its limited cold tolerance significantly impacts both the productivity and the geographical distribution of this species in higher-latitude areas. In this study, we employed RNA-Seq technology to delineate the transcriptomic alterations in Sesuvium plants exposed to low temperatures, thus advancing our comprehension of the molecular underpinnings of this physiological adaptation and root formation. Our findings demonstrated differential expression of 10,805, 16,389, and 10,503 genes in the low versus moderate temperature (LT vs. MT), moderate versus high temperature (MT vs. HT), and low versus high temperature (LT vs. HT) comparative analyses, respectively. Notably, the gene categories "structural molecule activity", "ribosome biogenesis", and "ribosome" were particularly enriched among the LT vs. HT-specific differentially expressed genes (DEGs). When synthesizing the insights from these three comparative studies, the principal pathways associated with the cold response mechanism were identified as "carbon fixation in photosynthetic organisms", "starch and sucrose metabolism", "plant hormone signal transduction", "glycolysis/gluconeogenesis", and "photosynthesis". In addition, we elucidated the involvement of auxin signaling pathways, adventitious root formation (ARF), lateral root formation (LRF), and novel genes associated with shoot system development in root formation. Subsequently, we constructed a network diagram to investigate the interplay between hormone levels and pivotal genes, thereby clarifying the regulatory pathways of plant root formation under low-temperature stress and isolating key genes instrumental in root development. This study has provided critical insights into the molecular mechanisms that facilitate the adaptation to cold stress and root formation in S. portulacastrum.

Abstract Image

Sesuvium portulacastrum L.的转录组分析揭示了低温胁迫下根系形成所涉及的关键基因和途径。
Sesuvium portulacastrum L.是一种多年生半生植物,广泛分布于热带和亚热带沿海地区。其有限的耐寒性严重影响了该物种在高纬度地区的生产力和地理分布。在这项研究中,我们采用 RNA-Seq 技术描述了暴露于低温环境中的 Sesuvium 植物的转录组变化,从而加深了我们对这种生理适应和根系形成的分子基础的理解。我们的研究结果表明,在低温与中温(LT vs. MT)、中温与高温(MT vs. HT)以及低温与高温(LT vs. HT)的比较分析中,分别有 10805、16389 和 10503 个基因的表达存在差异。值得注意的是,"结构分子活性"、"核糖体生物发生 "和 "核糖体 "等基因类别在低温与高温特异性差异表达基因(DEGs)中特别富集。综合上述三项比较研究的结果,与低温响应机制相关的主要途径被确定为 "光合生物的碳固定"、"淀粉和蔗糖代谢"、"植物激素信号转导"、"糖酵解/糖酮生成 "和 "光合作用"。此外,我们还阐明了参与根形成的辅助素信号通路、不定根形成(ARF)、侧根形成(LRF)以及与芽系统发育相关的新基因。随后,我们构建了一个网络图来研究激素水平与关键基因之间的相互作用,从而阐明了低温胁迫下植物根形成的调控途径,并分离出了根发育的关键基因。这项研究对促进 S. portulacastrum 适应低温胁迫和根形成的分子机制提供了重要启示。
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来源期刊
Plant Molecular Biology
Plant Molecular Biology 生物-生化与分子生物学
自引率
2.00%
发文量
95
审稿时长
1.4 months
期刊介绍: Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.
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