Muscle regeneration therapy using dedifferentiated fat cell (DFAT) for anal sphincter dysfunction.

IF 1.5 3区 医学 Q2 PEDIATRICS
Yusuke Kamidaki, Takashi Hosokawa, Naoko Abe, Eri Fujita, Bin Yamaoka, Kako Ono, Shumpei Goto, Tomohiko Kazama, Taro Matsumoto, Shuichiro Uehara
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Abstract

Purpose: We investigated the effects of mouse-derived DFAT on the myogenic differentiation of a mouse-derived myoblast cell line (C2C12) and examined the therapeutic effects of rat-derived DFAT on anal sphincter injury using a rat model.

Methods: C2C12 cells were cultured using DMEM and DFAT-conditioned medium (DFAT-CM), evaluating MyoD and Myogenin gene expression via RT-PCR. DFAT was locally administered to model rats with anorectal sphincter dysfunction 3 days post-CTX injection. Therapeutic effects were assessed through functional assessment, including anal pressure measurement using solid-state manometry pre/post-CTX, and on days 1, 3, 7, 10, 14, 17, and 21 post-DFAT administration. Histological evaluation involved anal canal excision on days 1, 3, 7, 14, and 21 after CTX administration, followed by hematoxylin-eosin staining.

Results: C2C12 cells cultured with DFAT-CM exhibited increased MyoD and Myogenin gene expression compared to control. Anal pressure measurements revealed early recovery of resting pressure in the DFAT-treated group. Histologically, DFAT-treated rats demonstrated an increase in mature muscle cells within newly formed muscle fibers on days 14 and 21 after CTX administration, indicating enhanced muscle tissue repair.

Conclusion: DFAT demonstrated the potential to enhance histological and functional muscle tissue repair. These findings propose DFAT as a novel therapeutic approach for anorectal sphincter dysfunction treatment.

Abstract Image

利用再分化脂肪细胞(DFAT)治疗肛门括约肌功能障碍的肌肉再生疗法。
目的:我们研究了鼠源性 DFAT 对鼠源性成肌细胞系(C2C12)成肌分化的影响,并利用大鼠模型研究了鼠源性 DFAT 对肛门括约肌损伤的治疗效果:方法:使用 DMEM 和 DFAT 条件培养基(DFAT-CM)培养 C2C12 细胞,通过 RT-PCR 评估 MyoD 和 Myogenin 基因的表达。在注射 CTX 后 3 天,对患有肛门直肠括约肌功能障碍的模型大鼠局部注射 DFAT。治疗效果通过功能评估进行评估,包括 CTX 注射前/后、DFAT 注射后第 1、3、7、10、14、17 和 21 天使用固态测压法测量肛门压力。组织学评估包括在服用 CTX 后的第 1、3、7、14 和 21 天进行肛管切除,然后进行苏木精-伊红染色:结果:与对照组相比,用 DFAT-CM 培养的 C2C12 细胞的 MyoD 和 Myogenin 基因表达增加。肛压测量显示,DFAT 处理组的静息压恢复较早。从组织学角度来看,在服用 CTX 后的第 14 天和第 21 天,DFAT 治疗组大鼠在新形成的肌肉纤维中显示出成熟肌肉细胞的增加,这表明肌肉组织修复能力增强:结论:DFAT具有增强肌肉组织组织学和功能修复的潜力。这些研究结果表明,DFAT 是治疗肛门直肠括约肌功能障碍的一种新型疗法。
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来源期刊
CiteScore
3.00
自引率
5.60%
发文量
215
审稿时长
3-6 weeks
期刊介绍: Pediatric Surgery International is a journal devoted to the publication of new and important information from the entire spectrum of pediatric surgery. The major purpose of the journal is to promote postgraduate training and further education in the surgery of infants and children. The contents will include articles in clinical and experimental surgery, as well as related fields. One section of each issue is devoted to a special topic, with invited contributions from recognized authorities. Other sections will include: -Review articles- Original articles- Technical innovations- Letters to the editor
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