Strigolactone-gibberellin crosstalk mediated by a distant silencer fine-tunes plant height in upland cotton.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-08-22 DOI:10.1016/j.molp.2024.08.007
Zailong Tian, Baojun Chen, Hongge Li, Xinxin Pei, Yaru Sun, Gaofei Sun, Zhaoe Pan, Panhong Dai, Xu Gao, Xiaoli Geng, Zhen Peng, Yinhua Jia, Daowu Hu, Liru Wang, Baoyin Pang, Ai Zhang, Xiongming Du, Shoupu He
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引用次数: 0

Abstract

Optimal plant height is crucial in modern agriculture, influencing lodging resistance and facilitating mechanized crop production. Upland cotton (Gossypium hirsutum) is the most important fiber crop globally; however, the genetic basis underlying plant height remains largely unexplored. In this study, we conducted a genome-wide association study to identify a major locus controlling plant height (PH1) in upland cotton. This locus encodes gibberellin 2-oxidase 1A (GhPH1) and features a 1133-bp structural variation (PAVPH1) located approximately 16 kb upstream. The presence or absence of PAVPH1 influences the expression of GhPH1, thereby affecting plant height. Further analysis revealed that a gibberellin-regulating transcription factor (GhGARF) recognizes and binds to a specific CATTTG motif in both the GhPH1 promoter and PAVPH1. This interaction downregulates GhPH1, indicating that PAVPH1 functions as a distant upstream silencer. Intriguingly, we found that DWARF53 (D53), a key repressor of the strigolactone (SL) signaling pathway, directly interacts with GhGARF to inhibit its binding to targets. Moreover, we identified a previously unrecognized gibberellin-SL crosstalk mechanism mediated by the GhD53-GhGARF-GhPH1/PAVPH1 module, which is crucial for regulating plant height in upland cotton. These findings shed light on the genetic basis and gene interaction network underlying plant height, providing valuable insights for the development of semi-dwarf cotton varieties through precise modulation of GhPH1 expression.

由遥远的沉默因子介导的赤霉内酯-赤霉素串扰微调了陆地棉的植株高度。
最佳株高在现代农业中至关重要,它影响着作物的抗倒伏性并促进机械化生产。陆地棉(Gossypium hirsutum)是全球最重要的纤维作物,但植株高度的遗传基础仍有待探索。在此,我们进行了一项全基因组关联研究(GWAS),以确定控制陆地棉株高的主要位点(PH1)。该基因座编码赤霉素 2-氧化酶 1A(GhPH1),其上游约 16 kb 处有一个 1,133 bp 长的结构变异(PAVPH1)。PAVPH1 的存在与否会导致 GhPH1 的不同表达,从而导致植株高度的变化。进一步分析发现,赤霉素调节转录因子(GhGARF)能识别 GhPH1 启动子和 PAVPH1 上的特定 "CATTTG "基序。这一结合事件下调了 GhPH1,表明 PAVPH1 起着遥远的上游沉默因子的作用。有趣的是,我们发现芪醇内酯(SL)信号通路的关键抑制因子 DWARF53(D53)与 GhGARF 直接相互作用,并抑制其与靶标的结合。此外,我们的研究还发现了一种以前未曾认识到的由 GhD53-GhGARF-GhPH1/PAVPH1 模块介导的 GA-SL 互作机制,该机制在调控陆地棉株高方面至关重要。这些发现揭示了植株高度的遗传基础和基因互作网络,为通过精确调控 GhPH1 的表达来培育半矮小棉花品种提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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